Literature DB >> 17604435

Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species.

Laura Baires-Varguez1, Alejandro Cruz-García, Lourdes Villa-Tanaka, Sergio Sánchez-García, Luis Alberto Gaitán-Cepeda, Luis Octavio Sánchez-Vargas, Guillermo Quindós, César Hernández-Rodríguez.   

Abstract

The objective of this work was to compare the usefulness of a randomly amplified polymorphic DNA (RAPD) assay to that of the ATB ID32C kit (bioMérieux, France) for identification of different species of Candida isolated from clinical specimens. The RAPD-PCR patterns obtained with OPE-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. RAPD with the OPE-18 primer is a very specific and sensitive method for identification of Candida glabrata, Candida guilliermondii, Candida tropicalis, Candida pelliculosa, Candida albicans, Candida krusei, and Candida lusitaniae.

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Year:  2007        PMID: 17604435     DOI: 10.1016/s1130-1406(07)70031-1

Source DB:  PubMed          Journal:  Rev Iberoam Micol        ISSN: 1130-1406            Impact factor:   1.044


  2 in total

1.  Multilocus microsatellite markers for molecular typing of Candida tropicalis isolates.

Authors:  Yuan Wu; Hai-jian Zhou; Jie Che; Wen-ge Li; Fu-ning Bian; Shuan-bao Yu; Li-juan Zhang; Jinxing Lu
Journal:  BMC Microbiol       Date:  2014-11-20       Impact factor: 3.605

2.  Identification and Antifungal Susceptibility Testing of Candida Species: A Comparison of Vitek-2 System with Conventional and Molecular Methods.

Authors:  Ravinder Kaur; Megh Singh Dhakad; Ritu Goyal; Absarul Haque; Gauranga Mukhopadhyay
Journal:  J Glob Infect Dis       Date:  2016 Oct-Dec
  2 in total

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