Literature DB >> 17603993

Dephospho-CoA kinase provides a rapid and sensitive radiochemical assay for coenzyme A and its thioesters.

Caryn Wadler1, John E Cronan.   

Abstract

A new approach to determine in vivo pools of coenzyme A (CoA) and short chain acyl-CoA thioesters is reported. The metabolites released by extraction with trichloroacetic acid are recovered and quantitatively dephosphorylated by treatment with shrimp alkaline phosphatase. Following phosphatase removal, the dephosphorylated CoA metabolites are quantitatively rephosphorylated by treatment with [gamma-33P]ATP plus a dephospho-CoA kinase. The resulting radioactive CoA metabolites are then separated by reverse-phase high-performance liquid chromatography and quantitated by scintillation counting. Due to the enzymatic radiophosphorylation, the assay is specific for CoA and its short chain thioesters and is sensitive to sub-picomole levels of these compounds.

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Year:  2007        PMID： 17603993      PMCID： PMC2669429          DOI： 10.1016/j.ab.2007.05.031

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  23 in total

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Authors:  Mark Shimazu; Leandro Vetcher; Jorge L Galazzo; Peter Licari; Daniel V Santi
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2.  High performance liquid chromatography stability study of malonyl-coenzyme A, using statistical experimental designs.

Authors:  B M De Spiegeleer; K Sintobin; J Desmet
Journal:  Biomed Chromatogr       Date:  1989-09       Impact factor: 1.902

3.  The effect of carbon and nitrogen sources on the level of metabolic intermediates in Escherichia coli.

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Journal:  J Biol Chem       Date:  1971-11       Impact factor: 5.157

4.  Acyl carrier protein. 8. Studies of acyl carrier protein and coenzyme A in Escherichia coli pantothenate or betaalanine auxotrophs.

Authors:  A W Alberts; P R Vagelos
Journal:  J Biol Chem       Date:  1966-11-25       Impact factor: 5.157

5.  Acyl carrier protein from Escherichia coli.

Authors:  C O Rock; J E Cronan
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

6.  High-pressure liquid chromatography--ultraviolet analysis of intracellular nucleotides.

Authors:  A L Pogolotti; D V Santi
Journal:  Anal Biochem       Date:  1982-11-01       Impact factor: 3.365

7.  Regulation of enzymes by fatty acyl coenzyme A. Interactions of short and long chain spin-labeled acyl-CoA with the acetyl-CoA site on pig heart citrate synthase.

Authors:  B C Hansel; G L Powell
Journal:  J Biol Chem       Date:  1984-02-10       Impact factor: 5.157

8.  Complete analysis of cellular nucleotides by two-dimensional thin layer chromatography.

Authors:  B R Bochner; B N Ames
Journal:  J Biol Chem       Date:  1982-08-25       Impact factor: 5.157

9.  Regulation of coenzyme A biosynthesis.

Authors:  S Jackowski; C O Rock
Journal:  J Bacteriol       Date:  1981-12       Impact factor: 3.490

10.  A re-examination of some properties of fatty acyl-CoA micelles.

Authors:  G L Powell; J R Grothusen; J K Zimmerman; C A Evans; W W Fish
Journal:  J Biol Chem       Date:  1981-12-25       Impact factor: 5.157
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  7 in total

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Journal:  Mol Microbiol       Date:  2011-02-24       Impact factor: 3.501

4.  Novel approach in LC-MS/MS using MRM to generate a full profile of acyl-CoAs: discovery of acyl-dephospho-CoAs.

Authors:  Qingling Li; Shenghui Zhang; Jessica M Berthiaume; Brigitte Simons; Guo-Fang Zhang
Journal:  J Lipid Res       Date:  2013-12-23       Impact factor: 5.922

5.  Liquid chromatography-tandem quadrupole mass spectrometry and comprehensive two-dimensional gas chromatography-time-of-flight mass spectrometry measurement of targeted metabolites of Methylobacterium extorquens AM1 grown on two different carbon sources.

Authors:  Song Yang; Martin Sadilek; Robert E Synovec; Mary E Lidstrom
Journal:  J Chromatogr A       Date:  2009-02-21       Impact factor: 4.759

6.  Sanhuang Xiexin Tang Ameliorates Type 2 Diabetic Rats via Modulation of the Metabolic Profiles and NF-κB/PI-3K/Akt Signaling Pathways.

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Review 7.  Dermatophyte Resistance to Antifungal Drugs: Mechanisms and Prospectus.

Authors:  Nilce M Martinez-Rossi; Tamires A Bitencourt; Nalu T A Peres; Elza A S Lang; Eriston V Gomes; Natalia R Quaresemin; Maíra P Martins; Lucia Lopes; Antonio Rossi
Journal:  Front Microbiol       Date:  2018-05-29       Impact factor: 5.640
  7 in total

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