Literature DB >> 17603991

An assay for thiaminase I in complex biological samples.

Jeremiah W Hanes1, Clifford E Kraft, Tadhg P Begley.   

Abstract

An alternative method for measuring thiaminase I activity in complex samples is described. This assay is based on the selective consumption of the highly chromophoric 4-nitrothiophenolate by thiaminase I, resulting in a large decrease in absorbance at 411nm. This new assay is simple and sensitive, and it requires only readily available chemicals and a visible region spectrophotometer. In addition, the assay is optimized for high-throughput analysis in a 96-well format with complex biological samples.

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Year:  2007        PMID： 17603991      PMCID： PMC2140240          DOI： 10.1016/j.ab.2007.06.001

Source DB:  PubMed          Journal:  Anal Biochem        ISSN： 0003-2697            Impact factor:   3.365


  14 in total

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Authors:  N L Kelleher; R B Nicewonger; T P Begley; F W McLafferty
Journal:  J Biol Chem       Date:  1997-12-19       Impact factor: 5.157

6.  Thiaminase I (42 kDa) heterogeneity, sequence refinement, and active site location from high-resolution tandem mass spectrometry.

Authors:  N L Kelleher; C A Costello; T P Begley; F W McLafferty
Journal:  J Am Soc Mass Spectrom       Date:  1995-10       Impact factor: 3.109

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Authors:  N Campobasso; C A Costello; C Kinsland; T P Begley; S E Ealick
Journal:  Biochemistry       Date:  1998-11-10       Impact factor: 3.162

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Authors:  N Campobasso; J Begun; C A Costello; T P Begley; S E Ealick
Journal:  Acta Crystallogr D Biol Crystallogr       Date:  1998-05-01

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Authors:  C A Costello; N L Kelleher; M Abe; F W McLafferty; T P Begley
Journal:  J Biol Chem       Date:  1996-02-16       Impact factor: 5.157
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  4 in total

1.  Construction of a thiamin sensor from the periplasmic thiamin binding protein.

Authors:  Jeremiah W Hanes; Debashree Chatterjee; Erika V Soriano; Steven E Ealick; Tadhg P Begley
Journal:  Chem Commun (Camb)       Date:  2010-12-02       Impact factor: 6.222

2.  Thiamine deficiency in Cambodian infants with and without beriberi.

Authors:  Debra Coats; Kelsey Shelton-Dodge; Kevanna Ou; Vannara Khun; Sommon Seab; Kimsan Sok; Chiva Prou; Silvia Tortorelli; Thomas P Moyer; Lisa E Cooper; Tadhg P Begley; Felicity Enders; Philip R Fischer; Mark Topazian
Journal:  J Pediatr       Date:  2012-06-14       Impact factor: 4.406

3.  A rapid method for assaying thiaminase I activity in diverse biological samples.

Authors:  Clifford E Kraft; Eric R L Gordon; Esther R Angert
Journal:  PLoS One       Date:  2014-03-27       Impact factor: 3.240

4.  Comparison of thiaminase activity in fish using the radiometric and 4-nitrothiophenol colorimetric methods.

Authors:  Dale C Honeyfield; Jeremiah W Hanes; Lisa Brown; Clifford E Kraft; Tadhg P Begley
Journal:  J Great Lakes Res       Date:  2010-12       Impact factor: 3.032
  4 in total

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