OBJECTIVE: To report a case of successful delivery of a healthy baby after intracytoplasmic sperm injection (ICSI) in a patient with no mature oocytes at the time of oocyte retrieval. DESIGN: Case report. SETTING: Department of Reproductive Medicine. PATIENT: After a controlled ovarian hyperstimulation cycle, a total of seven immature oocytes were collected. INTERVENTION(S): Medical management. MAIN OUTCOME MEASURE(S): The timing of polar body extrusion was checked every 2 hours, and ICSI was performed as soon as the first polar body was extruded. RESULT(S): Following incubation in culture medium, five oocytes reached the metaphase II stage within 8-8.5 hours. Three oocytes were fertilized after ICSI, and two of three cleaved embryos were transferred on day 3. The embryo transfer was followed by a single pregnancy and the delivery of a healthy baby. CONCLUSION: This case report demonstrates that embryos obtained from in vitro matured oocytes retain the developmental competence for full-term. It illustrates the importance of regularly monitoring the polar body extrusion when all collected oocytes are immature.
OBJECTIVE: To report a case of successful delivery of a healthy baby after intracytoplasmic sperm injection (ICSI) in a patient with no mature oocytes at the time of oocyte retrieval. DESIGN: Case report. SETTING: Department of Reproductive Medicine. PATIENT: After a controlled ovarian hyperstimulation cycle, a total of seven immature oocytes were collected. INTERVENTION(S): Medical management. MAIN OUTCOME MEASURE(S): The timing of polar body extrusion was checked every 2 hours, and ICSI was performed as soon as the first polar body was extruded. RESULT(S): Following incubation in culture medium, five oocytes reached the metaphase II stage within 8-8.5 hours. Three oocytes were fertilized after ICSI, and two of three cleaved embryos were transferred on day 3. The embryo transfer was followed by a single pregnancy and the delivery of a healthy baby. CONCLUSION: This case report demonstrates that embryos obtained from in vitro matured oocytes retain the developmental competence for full-term. It illustrates the importance of regularly monitoring the polar body extrusion when all collected oocytes are immature.
Authors: K E Hemmings; D Maruthini; S Vyjayanthi; J E Hogg; A H Balen; B K Campbell; H J Leese; H M Picton Journal: Hum Reprod Date: 2013-01-18 Impact factor: 6.918