Fanfan Zhou1, Wen Xu, Kunihiko Tanaka, Guofeng You. 1. Department of Pharmaceutics, Rutgers, The State University of New Jersey, 160 Frelinghuysen Road, Piscataway, New Jersey 08854, USA.
Abstract
PURPOSE: To compare the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells. MATERIALS AND METHODS: PDZ proteins PDZK1 and NHERF1 were transfected into kidney LLC-PK1 cells and placental BeWo cells expressing hOAT4 or hOAT4-Delta, which lacks the PDZ consensus binding site. The interaction of PDZK1 and NHERF1 with hOAT4 and hOAT4-Delta was investigated by measurement of [3H] estrone sulfate uptake, cell surface and total cell expression of hOAT4. RESULTS: PDZK1 and NHERF1 enhanced hOAT4 activity in LLC-PK1 cells by increasing the cell surface expression of the transporter. In contrasts, these two PDZ proteins had no effect on hOAT4 activity in BeWo cells. CONCLUSION: The interaction of PDZ proteins with hOAT4 may be cell-specific. In placenta, a different set of interacting proteins from PDZK1 and NHERF1 may be required to modulate hOAT4 activity.
PURPOSE: To compare the interaction of human organic anion transporter hOAT4 with PDZ proteins between kidney cells and placental cells. MATERIALS AND METHODS: PDZ proteins PDZK1 and NHERF1 were transfected into kidney LLC-PK1 cells and placental BeWo cells expressing hOAT4 or hOAT4-Delta, which lacks the PDZ consensus binding site. The interaction of PDZK1 and NHERF1 with hOAT4 and hOAT4-Delta was investigated by measurement of [3H] estrone sulfate uptake, cell surface and total cell expression of hOAT4. RESULTS:PDZK1 and NHERF1 enhanced hOAT4 activity in LLC-PK1 cells by increasing the cell surface expression of the transporter. In contrasts, these two PDZ proteins had no effect on hOAT4 activity in BeWo cells. CONCLUSION: The interaction of PDZ proteins with hOAT4 may be cell-specific. In placenta, a different set of interacting proteins from PDZK1 and NHERF1 may be required to modulate hOAT4 activity.
Authors: Bernhard Ugele; Marie V St-Pierre; Monika Pihusch; Andrew Bahn; Peer Hantschmann Journal: Am J Physiol Endocrinol Metab Date: 2002-10-29 Impact factor: 4.310
Authors: Pijun Wang; Jin J Wang; Yansen Xiao; John W Murray; Phyllis M Novikoff; Ruth Hogue Angeletti; George A Orr; Debin Lan; David L Silver; Allan W Wolkoff Journal: J Biol Chem Date: 2005-06-30 Impact factor: 5.157