BACKGROUND: Only a few infants develop acute bronchiolitis when exposed to respiratory syncytial virus (RSV), and host, environmental, and viral properties are probably all of importance in determining the severity of infection. METHODS: Microarray analysis was used to identify differentially expressed single genes and gene sets in cord blood from 5 infants hospitalized with RSV bronchiolitis versus cord blood from 5 control infants exposed to RSV without bronchiolitis during infancy. Quantitative real-time polymerase chain reaction (QRT-PCR) was performed on single genes in both the 5 infants selected for microarray analysis and 13 more infants hospitalized with the same disease. Gene set enrichment analysis (GSEA) was performed to identify differentially expressed gene sets within the microarray experiments. RESULTS: Microarray analysis identified 15 single genes to be significantly differentially expressed between case and control infants. Eleven of these genes were evaluated with QRT-PCR, and the genes FAM102A, TNFRSF25, and STMN3 were down-regulated in all but 1 of the 18 infants. A pathway involved in regulation of the actin cytoskeleton was found to be clearly down-regulated when analyzed with GSEA. CONCLUSIONS: FAM102A, TNFRSF25, and STMN3 and a pathway involved in regulation of the actin cytoskeleton are down-regulated in cord blood from infants hospitalized with RSV bronchiolitis.
BACKGROUND: Only a few infants develop acute bronchiolitis when exposed to respiratory syncytial virus (RSV), and host, environmental, and viral properties are probably all of importance in determining the severity of infection. METHODS: Microarray analysis was used to identify differentially expressed single genes and gene sets in cord blood from 5 infants hospitalized with RSV bronchiolitis versus cord blood from 5 control infants exposed to RSV without bronchiolitis during infancy. Quantitative real-time polymerase chain reaction (QRT-PCR) was performed on single genes in both the 5 infants selected for microarray analysis and 13 more infants hospitalized with the same disease. Gene set enrichment analysis (GSEA) was performed to identify differentially expressed gene sets within the microarray experiments. RESULTS: Microarray analysis identified 15 single genes to be significantly differentially expressed between case and control infants. Eleven of these genes were evaluated with QRT-PCR, and the genes FAM102A, TNFRSF25, and STMN3 were down-regulated in all but 1 of the 18 infants. A pathway involved in regulation of the actin cytoskeleton was found to be clearly down-regulated when analyzed with GSEA. CONCLUSIONS:FAM102A, TNFRSF25, and STMN3 and a pathway involved in regulation of the actin cytoskeleton are down-regulated in cord blood from infants hospitalized with RSV bronchiolitis.
Authors: Annemieke Schuurhof; Louis Bont; Jeroen L A Pennings; Hennie M Hodemaekers; Piet W Wester; Annemarie Buisman; Lia C G H de Rond; Myra N Widjojoatmodjo; Willem Luytjes; Jan L L Kimpen; Riny Janssen Journal: J Virol Date: 2010-06-30 Impact factor: 5.103
Authors: P M Tiwari; E Eroglu; S Boyoglu-Barnum; Q He; G A Willing; K Vig; V A Dennis; S R Singh Journal: J Microsc Date: 2013-11-19 Impact factor: 1.758
Authors: Asuncion Mejias; Blerta Dimo; Nicolas M Suarez; Carla Garcia; M Carmen Suarez-Arrabal; Tuomas Jartti; Derek Blankenship; Alejandro Jordan-Villegas; Monica I Ardura; Zhaohui Xu; Jacques Banchereau; Damien Chaussabel; Octavio Ramilo Journal: PLoS Med Date: 2013-11-12 Impact factor: 11.069
Authors: Lien Anh Ha Do; Johann Pellet; H Rogier van Doorn; Anh Tuan Tran; Bach Hue Nguyen; Thi Thu Loan Tran; Quynh Huong Tran; Quoc Bao Vo; Nguyen Anh Tran Dac; Hong Nhien Trinh; Thi Thanh Hai Nguyen; Bao Tinh Le Binh; Huu Mai Khanh Nguyen; Minh Tien Nguyen; Quang Tung Thai; Thanh Vu Vo; Ngoc Quang Minh Ngo; Thi Kim Huyen Dang; Ngoc Huong Cao; Thu Van Tran; Lu Viet Ho; Bertrand De Meulder; Charles Auffray; Jorrit-Jan Hofstra; Jeremy Farrar; Juliet E Bryant; Menno de Jong; Martin L Hibberd Journal: J Infect Dis Date: 2017-12-27 Impact factor: 5.226