BACKGROUND: The Beclin 1 gene binds to the apoptosis-inhibiting protein Bcl-2. The expression and function of the Beclin 1 gene in four colorectal cancer cell lines (HT29, DLD-1, SW480, SW620) was investigated. MATERIALS AND METHODS: The expression of Beclin 1 mRNA was examined by reverse transcription-polymerase chain reaction (RT-PCR), the cell growth rate of a stable cell line established after transfection of the Beclin 1 gene into low Beclin 1-expressing HT29 colon cancer cells. In addition, changes in cell cycle were investigated by flow cytometry, and the expression levels of cell cycle related proteins in the Beclin 1 transfected colon cancer cell line were assessed. RESULTS: The expression level of Beclin 1 mRNA in the colon cancer cell lines was variable. Transfection of the low Beclin 1 gene-expressing colon cancer cell line with the Beclin 1 gene resulted in cell growth inhibition. The cell cycle analysis showed that the percentage of GJ-phase cells was significantly higher in Beclin 1 transfectants than in mock transfected cells. The expression levels of cyclin E and phosphorylated Rb were decreased. CONCLUSION: These results indicate that Beclin 1 can inhibit the growth of colorectal cancer cells.
BACKGROUND: The Beclin 1 gene binds to the apoptosis-inhibiting protein Bcl-2. The expression and function of the Beclin 1 gene in four colorectal cancer cell lines (HT29, DLD-1, SW480, SW620) was investigated. MATERIALS AND METHODS: The expression of Beclin 1 mRNA was examined by reverse transcription-polymerase chain reaction (RT-PCR), the cell growth rate of a stable cell line established after transfection of the Beclin 1 gene into low Beclin 1-expressing HT29 colon cancer cells. In addition, changes in cell cycle were investigated by flow cytometry, and the expression levels of cell cycle related proteins in the Beclin 1transfected colon cancer cell line were assessed. RESULTS: The expression level of Beclin 1 mRNA in the colon cancer cell lines was variable. Transfection of the low Beclin 1 gene-expressing colon cancer cell line with the Beclin 1 gene resulted in cell growth inhibition. The cell cycle analysis showed that the percentage of GJ-phase cells was significantly higher in Beclin 1 transfectants than in mock transfected cells. The expression levels of cyclin E and phosphorylated Rb were decreased. CONCLUSION: These results indicate that Beclin 1 can inhibit the growth of colorectal cancer cells.
Authors: Heather B Roesly; Mohammad R Khan; Hwu Dau Rw Chen; Kimberly A Hill; Nirushan Narendran; George S Watts; Xiaoxin Chen; Katerina Dvorak Journal: Am J Physiol Gastrointest Liver Physiol Date: 2012-02-02 Impact factor: 4.052
Authors: Ellen Wirawan; Tom Vanden Berghe; Saskia Lippens; Patrizia Agostinis; Peter Vandenabeele Journal: Cell Res Date: 2011-09-13 Impact factor: 25.617