P Picart1, P Diaz, F I J Pastor. 1. Department of Microbiology, Faculty of Biology, University of Barcelona, Spain.
Abstract
AIMS: To isolate new fungal strains from subtropical soils and to identify those that produce high cellulase activity. To select microbial strains producing thermostable cellulases with potential application in industry. METHODS AND RESULTS: The new strains Penicillium sp. CR-316 and Penicillium sp. CR-313 have been identified and selected because they secreted a high level of cellulase in media supplemented with rice straw. Analysis by sodium dodecyl sulfate polyacrylamide gel electrophoresis, isoelectric focussing and zymography showed that the studied strains secreted multiple enzymes that hydrolyse cellulose. Cellulase activity of Penicillium sp. CR-316, the strain showing higher production, was analysed. Optimum temperature and pH of carboxymethyl cellulase activity were 65 degrees C and pH 4.5, respectively. Activity remained stable after incubation at 60 degrees C and pH 4.5 for 3 h. CONCLUSIONS: Fungal strains that secrete high levels of cellulase activity have been characterized and selected from soil. The isolated strains have complex sets of enzymes for cellulose degradation. Crude cellulase produced by Penicillium sp. CR-316 showed activity and stability at high temperature. SIGNIFICANCE AND IMPACT OF THE STUDY: Two fungal strains with biotechnological potential have been isolated. The strains secrete high levels of cellulase, and one of them, Penicillium sp. CR-316, produces a thermostable cellulase, that makes it a good candidate for industrial applications.
AIMS: To isolate new fungal strains from subtropical soils and to identify those that produce high cellulase activity. To select microbial strains producing thermostable cellulases with potential application in industry. METHODS AND RESULTS: The new strains Penicillium sp. CR-316 and Penicillium sp. CR-313 have been identified and selected because they secreted a high level of cellulase in media supplemented with rice straw. Analysis by sodium dodecyl sulfatepolyacrylamide gel electrophoresis, isoelectric focussing and zymography showed that the studied strains secreted multiple enzymes that hydrolyse cellulose. Cellulase activity of Penicillium sp. CR-316, the strain showing higher production, was analysed. Optimum temperature and pH of carboxymethyl cellulase activity were 65 degrees C and pH 4.5, respectively. Activity remained stable after incubation at 60 degrees C and pH 4.5 for 3 h. CONCLUSIONS: Fungal strains that secrete high levels of cellulase activity have been characterized and selected from soil. The isolated strains have complex sets of enzymes for cellulose degradation. Crude cellulase produced by Penicillium sp. CR-316 showed activity and stability at high temperature. SIGNIFICANCE AND IMPACT OF THE STUDY: Two fungal strains with biotechnological potential have been isolated. The strains secrete high levels of cellulase, and one of them, Penicillium sp. CR-316, produces a thermostable cellulase, that makes it a good candidate for industrial applications.
Authors: Polyanna N Herculano; D M M Lima; M J S Fernandes; R P Neves; C M Souza-Motta; A L F Porto Journal: Curr Microbiol Date: 2011-01-29 Impact factor: 2.188
Authors: Carl J Yeoman; Yejun Han; Dylan Dodd; Charles M Schroeder; Roderick I Mackie; Isaac K O Cann Journal: Adv Appl Microbiol Date: 2010-03-06 Impact factor: 5.086
Authors: Vanessa de Cássia Teixeira da Silva; Amanda Lais de Souza Coto; Rafael de Carvalho Souza; Marcello Bertoldi Sanchez Neves; Eleni Gomes; Gustavo Orlando Bonilla-Rodriguez Journal: Biochem Res Int Date: 2016-05-08
Authors: Karin Vega; Gretty K Villena; Victor H Sarmiento; Yvette Ludeña; Nadia Vera; Marcel Gutiérrez-Correa Journal: Biotechnol Res Int Date: 2012-11-19