PURPOSE: To evaluate the cysticidal effect of polyhexamethylene biguanide (PHMB) and chlorhexidine on Acanthamoeba and its toxic effect on cultured human keratocytes. METHODS: Each well of a twofold-diluted Acanthamoeba cyst-containing suspension of 5 x 10(4) cysts/mL was treated with PHMB and chlorhexidine for 8, 24, and 48 hours to determine the minimal cysticidal concentration (MCC) of each disinfectant. Human corneal keratocytes (5 x 10(4) cells/mL) were exposed to PHMB and chlorhexidine for the same time to determine the survival rate of keratocytes. Inverted phase-contrast and electron microscopy were used to observe the morphologic changes. RESULTS: The mean MCC of PHMB for 8, 24, and 48 hours was 9.42, 5.62, and 2.37 microg/mL, respectively. The mean MCC of chlorhexidine for 8, 24, and 48 hours was 24.32, 10.02, and 7.02 microg/mL, respectively. The respective survival rate of keratocytes at the MCC was 91.7%, 64.6%, and 49.7% for PHMB and 95.7%, 90.6%, and 78.1% for chlorhexidine, respectively. The cysts and keratocytes showed more damaged appearances after treatment with PHMB than chlorhexidine. CONCLUSIONS: PHMB and chlorhexidine showed a similar amoebicidal efficacy. However, PHMB seemed to be more toxic to keratocytes than chlorhexidine.
PURPOSE: To evaluate the cysticidal effect of polyhexamethylene biguanide (PHMB) and chlorhexidine on Acanthamoeba and its toxic effect on cultured human keratocytes. METHODS: Each well of a twofold-diluted Acanthamoeba cyst-containing suspension of 5 x 10(4) cysts/mL was treated with PHMB and chlorhexidine for 8, 24, and 48 hours to determine the minimal cysticidal concentration (MCC) of each disinfectant. Human corneal keratocytes (5 x 10(4) cells/mL) were exposed to PHMB and chlorhexidine for the same time to determine the survival rate of keratocytes. Inverted phase-contrast and electron microscopy were used to observe the morphologic changes. RESULTS: The mean MCC of PHMB for 8, 24, and 48 hours was 9.42, 5.62, and 2.37 microg/mL, respectively. The mean MCC of chlorhexidine for 8, 24, and 48 hours was 24.32, 10.02, and 7.02 microg/mL, respectively. The respective survival rate of keratocytes at the MCC was 91.7%, 64.6%, and 49.7% for PHMB and 95.7%, 90.6%, and 78.1% for chlorhexidine, respectively. The cysts and keratocytes showed more damaged appearances after treatment with PHMB than chlorhexidine. CONCLUSIONS:PHMB and chlorhexidine showed a similar amoebicidal efficacy. However, PHMB seemed to be more toxic to keratocytes than chlorhexidine.
Authors: Andrea Cruzat; Wolfgang A Schrems; Laura M Schrems-Hoesl; Bernardo M Cavalcanti; Neda Baniasadi; Deborah Witkin; Deborah Pavan-Langston; Reza Dana; Pedram Hamrah Journal: Invest Ophthalmol Vis Sci Date: 2015-10 Impact factor: 4.799
Authors: Carmen M Martín-Navarro; Atteneri López-Arencibia; Ines Sifaoui; María Reyes-Batlle; Basilio Valladares; Enrique Martínez-Carretero; José E Piñero; Sutherland K Maciver; Jacob Lorenzo-Morales Journal: Antimicrob Agents Chemother Date: 2015-03-02 Impact factor: 5.191
Authors: C M Martín-Navarro; A López-Arencibia; F Arnalich-Montiel; B Valladares; J E Piñero; J Lorenzo-Morales Journal: Graefes Arch Clin Exp Ophthalmol Date: 2013-05-19 Impact factor: 3.117