BACKGROUND/AIMS: Recent studies have demonstrated that refluxed duodenal contents cause esophageal carcinoma in rats without exposure to carcinogens. Oxidative damage has long been related to carcinogenesis in human cancers and animal cancer models. The purpose here was to investigate the pathogenesis of esophageal cancer in the experiment of chronic duodenal content reflux without carcinogen. METHODOLOGY: Thirty 8-week-old male Wistar rats were exposed to duodenal content esophageal reflux. All animals underwent an esophagoduodenal anastomosis (EDA) with total gastrectomy in order to produce chronic esophagitis. In 10 rats the sham operation induced a midline laparotomy alone (Control). A total of 37 of 40 (92.5%) rats completed the study. In the EDA group, 27 (90%) rats completed the study. In the control group, 10 (100%) rats completely the study. They were sacrificed at the 35th week. Their esophagi were examined for the presence of cancer, Barrett's esophagus (BE), columnar line epithelium (CLE) and oxidative stress. RESULTS: After 35 weeks of reflux, columnar dysplasia and squamous carcinoma were found. PCNA labeling index was higher in dysplastic and cancer tissue than that of normal. To discover the role of oxidative stress and radical scavenger capacity in the malignant transformation of Barrett's esophagus, we measured Malondialdehyde (MDA), Superoxide dismutase (SOD) activity and Glutathione (GSH) content in EDA rats. Mucosal MDA levels were significantly increased in EDA groups compared with the normal controls. GSH and SOD levels were significantly decreased in EDA group compared with the normal control group. CONCLUSIONS: We proposed that oxidative damage plays an important role in the formation of esophageal cancer with EDA model.
BACKGROUND/AIMS: Recent studies have demonstrated that refluxed duodenal contents cause esophageal carcinoma in rats without exposure to carcinogens. Oxidative damage has long been related to carcinogenesis in humancancers and animal cancer models. The purpose here was to investigate the pathogenesis of esophageal cancer in the experiment of chronic duodenal content reflux without carcinogen. METHODOLOGY: Thirty 8-week-old male Wistar rats were exposed to duodenal content esophageal reflux. All animals underwent an esophagoduodenal anastomosis (EDA) with total gastrectomy in order to produce chronic esophagitis. In 10 rats the sham operation induced a midline laparotomy alone (Control). A total of 37 of 40 (92.5%) rats completed the study. In the EDA group, 27 (90%) rats completed the study. In the control group, 10 (100%) rats completely the study. They were sacrificed at the 35th week. Their esophagi were examined for the presence of cancer, Barrett's esophagus (BE), columnar line epithelium (CLE) and oxidative stress. RESULTS: After 35 weeks of reflux, columnar dysplasia and squamous carcinoma were found. PCNA labeling index was higher in dysplastic and cancer tissue than that of normal. To discover the role of oxidative stress and radical scavenger capacity in the malignant transformation of Barrett's esophagus, we measured Malondialdehyde (MDA), Superoxide dismutase (SOD) activity and Glutathione (GSH) content in EDArats. Mucosal MDA levels were significantly increased in EDA groups compared with the normal controls. GSH and SOD levels were significantly decreased in EDA group compared with the normal control group. CONCLUSIONS: We proposed that oxidative damage plays an important role in the formation of esophageal cancer with EDA model.
Authors: Jianping Kong; Kelly A Whelan; Dorottya Laczkó; Brendan Dang; Angeliz Caro Monroig; Ali Soroush; John Falcone; Ravi K Amaravadi; Anil K Rustgi; Gregory G Ginsberg; Gary W Falk; Hiroshi Nakagawa; John P Lynch Journal: Mol Carcinog Date: 2015-09-16 Impact factor: 4.784
Authors: Heng Lu; Ajaz A Bhat; Dunfa Peng; Zheng Chen; Shoumin Zhu; Jun Hong; Selma Maacha; Jin Yan; David J Robbins; M Kay Washington; Abbes Belkhiri; Wael El-Rifai Journal: Cancer Res Date: 2019-07-15 Impact factor: 12.701
Authors: Jun Hong; Zheng Chen; Dunfa Peng; Alexander Zaika; Frank Revetta; M Kay Washington; Abbes Belkhiri; Wael El-Rifai Journal: Oncotarget Date: 2016-03-29