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Literature DB >> 175895

Demonstration of cyclic AMP in bone cells by immuno-histochemical methods.

Z Davidovitch, P C Montgomery, O Eckerdal, G T Gustafson.

Abstract

By utilizing immunohistochemical procedures it was possible to locate bone cells that contained adenosine 3'5'-monophosphate (cyclic AMP) in frozen, undecalcified cat jaw sections. An immunoglobulin-enzyme bridge method was found to be superior to other techniques, including immunofluorescence. Differences in staining pattern were found between bone cells of kittens and young adult cats, indicating different metabolic levels related to cellular maturity, and different intracellular compartmentalization of cyclic AMP in the two age groups.

Entities: Chemical Species

Mesh：

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Year: 1976 PMID： 175895 DOI： 10.1007/bf02564013

Source DB: PubMed Journal: Calcif Tissue Res ISSN： 0008-0594

12 in total

1. The measurement of adenosine 3,5-monophosphate in bone.

Authors: J Shanfeld; I Shapiro; Z Davidovitch
Journal: Anal Biochem Date: 1975-06 Impact factor: 3.365

2. Enzyme-labeled antibodies: preparation and application for the localization of antigens.

Authors: P K Nakane; G B Pierce
Journal: J Histochem Cytochem Date: 1966-12 Impact factor: 2.479

3. Radioimmunoassay for the measurement of cyclic nucleotides.

Authors: A L Steiner; R E Wehmann; C W Parker; D M Kipnis
Journal: Adv Cyclic Nucleotide Res Date: 1972

4. Cyclic guanosine and adenosine 3',5'-monophosphates in canine thyroid: localization by immunofluorescence.

Authors: E F Fallon; R Agrawal; E Furth; A L Steiner; R Cowden
Journal: Science Date: 1974-06-07 Impact factor: 47.728

5. A method for combined microradiographic and histological analysis of non-decalcified hard tissues.

Authors: O Eckerdal
Journal: Acta Odontol Scand Date: 1972-09 Impact factor: 2.331

6. An immunoglobulin-enzyme bridge method for localizing tissue antigens.

Authors: T E Mason; R F Phifer; S S Spicer; R A Swallow; R B Dreskin
Journal: J Histochem Cytochem Date: 1969-09 Impact factor: 2.479

7. Selective enzyme purification by affinity chromatography.

Authors: P Cuatrecasas; M Wilchek; C B Anfinsen
Journal: Proc Natl Acad Sci U S A Date: 1968-10 Impact factor: 11.205

8. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

Authors: R C Graham; M J Karnovsky
Journal: J Histochem Cytochem Date: 1966-04 Impact factor: 2.479

Demonstration of cyclic AMP in bone cells by immuno-histochemical methods.

1. The measurement of adenosine 3,5-monophosphate in bone.

2. Enzyme-labeled antibodies: preparation and application for the localization of antigens.

3. Radioimmunoassay for the measurement of cyclic nucleotides.

4. Cyclic guanosine and adenosine 3',5'-monophosphates in canine thyroid: localization by immunofluorescence.

5. A method for combined microradiographic and histological analysis of non-decalcified hard tissues.

6. An immunoglobulin-enzyme bridge method for localizing tissue antigens.

7. Selective enzyme purification by affinity chromatography.

8. The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.

9. Radioimmunoassay for the measurement of adenosine 3',5'-cyclic phosphate.

10. Adenosine 3',5'-monophosphate is localized in cerebellar neurons: immunofluorescence evidence.

1. Cellular localization of cyclic AMP in periodontal tissues during experimental tooth movement in cats.

2. Cellular localization and concentration of bone cyclic nucleotides in response to acute PTE administration.

3. Guanosine 3',5'-monophosphate in bone: microscopic visualization by an immuno-histochemical technique.