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Literature DB >> 17588144

Designing a highly efficient refolding system for alkaline phosphatase using combination of cyclodextrin and Mg2+ ion.

Fariba Khodagholi¹, Razieh Yazdanparast.

Abstract

Two different folding aids including alpha-cyclodextrin and Mg2+ ions were applied to alkaline phosphatase refolding. The refolding yield depending on concentration of each of the refolding agents reached to 55 and 52% in the presence of 100 mM alpha-cyclodextrin and/or 5 mM Mg2+ ions, respectively. However, the refolding yield, mediated by combination of alpha-cyclodextrin and Mg2+ ions, was more than 96%. Replacement of Mg2+ ion with other type of ions which interact with alpha-cyclodextrin interfere with the function of cyclodextrin resulting in low refolding yields.

Entities: Chemical Gene

Mesh：

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Year: 2008 PMID： 17588144 DOI： 10.1007/s10930-006-9021-8

Source DB: PubMed Journal: Protein J ISSN： 1572-3887 Impact factor: 2.371

16 in total

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Journal: Semin Cell Dev Biol Date: 2000-02 Impact factor: 7.727

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Journal: Nature Date: 1996-06-13 Impact factor: 49.962

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Authors: M Bortolato; F Besson; B Roux
Journal: Proteins Date: 1999-11-01

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Journal: Biochim Biophys Acta Date: 1995-04-27

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Authors: G Cathala; C Brunel
Journal: J Biol Chem Date: 1975-08-10 Impact factor: 5.157

Designing a highly efficient refolding system for alkaline phosphatase using combination of cyclodextrin and Mg2+ ion.

Review 1. Roles of molecular chaperones in cytoplasmic protein folding.

2. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase.

Review 3. Molecular chaperones in cellular protein folding.

4. Alkaline phosphatase of Escherichia coli. Composition.

5. Role of metal ions on the secondary and quaternary structure of alkaline phosphatase from bovine intestinal mucosa.

6. Thermal and pH stabilities of alkaline phosphatase from bovine intestinal mucosa: a FTIR study.

7. Bovine kidney alkaline phosphatase. Catalytic properties, subunit interactions in the catalytic process, and mechanism of Mg2+ stimulation.

8. Kinetic aspects of alkaline phosphatase refolding in the presence of alpha-cyclodextrin.

9. Anion-induced refolding of human serum albumin under low pH conditions.

10. Study of refolding of calf intestinal alkaline phosphatase.

1. Designing a highly efficient chemical chaperone system using chitosan-coated alginate.

2. Evaluation of chaperone-like activity of alginate: microcapsule and water-soluble forms.

3. Cloning and Expression of Functional Reteplase in Escherichia coli TOP10.