| Literature DB >> 17584762 |
Valentina Gelfanova1, Richard E Higgs, Robert A Dean, David M Holtzman, Martin R Farlow, Eric R Siemers, Amechand Boodhoo, Yue-Wei Qian, Xiaohua He, Zhaoyan Jin, Deborah L Fisher, Karen L Cox, John E Hale.
Abstract
Immunoprecipitation (IP) combined with matrix-assisted laser desorption ionization (MALDI) time of flight (Tof) mass spectrometry has been used to develop quantitative assays for amyloid-beta (Abeta) peptides in cerebrospinal fluid (CSF). Inclusion of (15)N labelled standard peptides allows for absolute quantification of multiple Abeta isoforms in individual samples. Characterization of variability associated with all steps of the assay indicated that the IP step is the single largest contributor to overall variability. Optimization of the assay resulted in overall coefficient of variation <or=8% with high agreement to an Abeta(1-40) and Abeta(1-42) ELISA assay. Application of the MALDI-Tof assay to CSF obtained from healthy volunteers and Alzheimer's disease patients indicated statistically significant 43% lower levels of Abeta(1-42) in the AD group (P = 0.0025).Entities:
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Year: 2007 PMID： 17584762 DOI： 10.1093/bfgp/elm010
Source DB: PubMed Journal: Brief Funct Genomic Proteomic ISSN： 1473-9550