OBJECTIVES: To assess the effects of intermittent limb compression on arterial collateral formation in a rabbit-model. DESIGN: Animal study. MATERIAL AND METHODS: New Zealand rabbits (n=11), aged 2-years, weight of at least 4.0 kg, underwent bilateral superficial femoral artery ligation. In ten of these, the experimental leg underwent 60 minutes of daily intermittent compression for a ten week period with 3 sec/90 mmHg pressure inflation and a cycle of 3 times per minute. The contra-lateral limbs were not treated. At the end of the ten-week period, high-resolution angiograms were obtained by barium infusion into the aorta. The angiograms were analyzed in a blinded manner and the number of collateral arteries larger than 100 microns, was counted. Following perfusion-fixation, histological specimens of transverse sections of the compressed semi-membranous muscle were examined. RESULTS: The compressed limbs demonstrated a significantly (8.1+/-.87 vs 6.0+/-.97; p<0.005) greater number of collateral vessels, ranging in size from 100-700 microns, as compared to the control sides. The mean size of collaterals in the compressed limbs was not significantly different (0.33+/-0.17 vs 0.31+/-0.16). Microscopic examination of the collaterals confirmed remodeling by a typical neo-intima consisting of 6-7 cell-layers. CONCLUSIONS: Intermittent limb compression increases the number of angiographical collateral arteries.
OBJECTIVES: To assess the effects of intermittent limb compression on arterial collateral formation in a rabbit-model. DESIGN: Animal study. MATERIAL AND METHODS:New Zealand rabbits (n=11), aged 2-years, weight of at least 4.0 kg, underwent bilateral superficial femoral artery ligation. In ten of these, the experimental leg underwent 60 minutes of daily intermittent compression for a ten week period with 3 sec/90 mmHg pressure inflation and a cycle of 3 times per minute. The contra-lateral limbs were not treated. At the end of the ten-week period, high-resolution angiograms were obtained by barium infusion into the aorta. The angiograms were analyzed in a blinded manner and the number of collateral arteries larger than 100 microns, was counted. Following perfusion-fixation, histological specimens of transverse sections of the compressed semi-membranous muscle were examined. RESULTS: The compressed limbs demonstrated a significantly (8.1+/-.87 vs 6.0+/-.97; p<0.005) greater number of collateral vessels, ranging in size from 100-700 microns, as compared to the control sides. The mean size of collaterals in the compressed limbs was not significantly different (0.33+/-0.17 vs 0.31+/-0.16). Microscopic examination of the collaterals confirmed remodeling by a typical neo-intima consisting of 6-7 cell-layers. CONCLUSIONS: Intermittent limb compression increases the number of angiographical collateral arteries.
Authors: Bruno T Roseguini; Arturo A Arce-Esquivel; Sean C Newcomer; M H Laughlin Journal: Am J Physiol Regul Integr Comp Physiol Date: 2011-09-28 Impact factor: 3.619
Authors: C Schwahn-Schreiber; F X Breu; E Rabe; I Buschmann; W Döller; G R Lulay; A Miller; E Valesky; S Reich-Schupke Journal: Hautarzt Date: 2018-08 Impact factor: 0.751
Authors: Bruno T Roseguini; S Mehmet Soylu; Jeffrey J Whyte; H T Yang; Sean Newcomer; M Harold Laughlin Journal: Am J Physiol Heart Circ Physiol Date: 2010-03-26 Impact factor: 4.733
Authors: Bruno T Roseguini; Arturo A Arce-Esquivel; Sean C Newcomer; Hsiao T Yang; Ronald Terjung; M H Laughlin Journal: J Appl Physiol (1985) Date: 2012-02-23