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Literature DB >> 17581802

Purification of bovine hemoglobin via fast performance liquid chromatography.

Abstract

Bovine hemoglobin (bHb) was purified from bovine red blood cells (bRBCs) via anion exchange chromatography preceded by dialysis. This is a fast and effective way to obtain bHb from bRBCs using Q Sepharose XL, a strong anion exchange resin. This resin had double the binding capacity for bHb compared to three other anion exchange resins that were studied in this work. Methemoglobin levels remained below 2% with bHb concentrations between 0.7 and 1.7 mM. The high purity of bHb was confirmed via SDS-PAGE and size exclusion chromatography (SEC).

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Year: 2007 PMID： 17581802 PMCID： PMC1994826 DOI： 10.1016/j.jchromb.2007.05.040

Source DB: PubMed Journal: J Chromatogr B Analyt Technol Biomed Life Sci ISSN： 1570-0232 Impact factor: 3.205

22 in total

1. Standardization of hemoglobinometry. II. The hemiglobincyanide method.

Authors: E van KAMPEN; W G ZIJLSTRA
Journal: Clin Chim Acta Date: 1961-07 Impact factor: 3.786

2. Stability of liposome encapsulated hemoglobin dispersions.

Authors: Dian R Arifin; Andre F Palmer
Journal: Artif Cells Blood Substit Immobil Biotechnol Date: 2005

3. Physical properties and stability mechanisms of poly(ethylene glycol) conjugated liposome encapsulated hemoglobin dispersions.

Authors: Dian R Arifin; Andre F Palmer
Journal: Artif Cells Blood Substit Immobil Biotechnol Date: 2005

4. Preparation of human hemoglobin Ao for possible use as a blood substitute.

Authors: S M Christensen; F Medina; R W Winslow; S M Snell; A Zegna; M A Marini
Journal: J Biochem Biophys Methods Date: 1988-10

5. Studies on the heterogeneity of hemoglobin. 13. Chromatography of various human and animal hemoglobin types on DEAE-Sephadex.

Authors: A M Dozy; E F Kleihauer; T H Huisman
Journal: J Chromatogr Date: 1968-02-20

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors: U K Laemmli
Journal: Nature Date: 1970-08-15 Impact factor: 49.962

7. International Committee for Standardization in Haematology. Recommendations for reference method for haemoglobinometry in human blood (ICSH standard EP 6/2: 1977) and specifications for international haemiglobincyanide reference preparation (ICSH standard EP 6/3: 1977).

Authors:
Journal: J Clin Pathol Date: 1978-02 Impact factor: 3.411

8. Preparation of blood hemoglobins of vertebrates.

Authors: A Riggs
Journal: Methods Enzymol Date: 1981 Impact factor: 1.600

9. Photopolymerization of bovine hemoglobin entrapped nanoscale hydrogel particles within liposomal reactors for use as an artificial blood substitute.

Authors: Jaqunda N Patton; Andre F Palmer
Journal: Biomacromolecules Date: 2005 Jan-Feb Impact factor: 6.988

10. Effect of Cl- and H+ on the oxygen binding properties of glutaraldehyde-polymerized bovine hemoglobin-based blood substitutes.

Authors: Julie H Eike; Andre F Palmer
Journal: Biotechnol Prog Date: 2004 Sep-Oct

8 in total

Purification of bovine hemoglobin via fast performance liquid chromatography.

1. Standardization of hemoglobinometry. II. The hemiglobincyanide method.

2. Stability of liposome encapsulated hemoglobin dispersions.

3. Physical properties and stability mechanisms of poly(ethylene glycol) conjugated liposome encapsulated hemoglobin dispersions.

4. Preparation of human hemoglobin Ao for possible use as a blood substitute.

5. Studies on the heterogeneity of hemoglobin. 13. Chromatography of various human and animal hemoglobin types on DEAE-Sephadex.

6. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

7. International Committee for Standardization in Haematology. Recommendations for reference method for haemoglobinometry in human blood (ICSH standard EP 6/2: 1977) and specifications for international haemiglobincyanide reference preparation (ICSH standard EP 6/3: 1977).

8. Preparation of blood hemoglobins of vertebrates.

9. Photopolymerization of bovine hemoglobin entrapped nanoscale hydrogel particles within liposomal reactors for use as an artificial blood substitute.

10. Effect of Cl- and H+ on the oxygen binding properties of glutaraldehyde-polymerized bovine hemoglobin-based blood substitutes.

1. The effects of temperature and pH on secondary structure and antioxidant activity of Crocodylus siamensis hemoglobin.

2. Purification of Lumbricus terrestris erythrocruorin (LtEc) with anion exchange chromatography.

3. Purification of hemoglobin by tangential flow filtration with diafiltration.

4. Preparation of ultrapure bovine and human hemoglobin by anion exchange chromatography.

5. Tangential flow filtration of hemoglobin.

6. Site-selective glycosylation of hemoglobin on Cys beta93.

7. Progressive Saturation Improves the Encapsulation of Functional Proteins in Nanoscale Polymer Vesicles.

8. A Smart Capsule System for Automated Detection of Intestinal Bleeding Using HSL Color Recognition.