Literature DB >> 17578679

Isoelectric focusing and ELISA evaluation of a Blastomyces dermatitidis human isolate.

Jack F Shurley1, Gene M Scalarone.   

Abstract

Blastomyces dermatitidis is a dimorphic fungal organism and the causative agent of blastomycosis. This organism is endemic east of the Mississippi river as is the fungal organism Histoplasma capsulatum. This study was performed to determine if sensitive and specific antigens from the B. dermatitidis yeast phase lysate (human isolate 592) could be separated using isoelectric focusing (IEF) to eliminate antigens that are cross-reactive with H. capsulatum. Indirect enzyme linked immunosorbent assays were performed to test for reactivity and cross-reactivity and indicate that certain fractions (4-6) were highly reactive. Fraction 16 exhibited a high degree of cross-reactivity with H. capsulatum. This study indicates that IEF may be a useful method for the separation of B. dermatitidis proteins.

Entities:  

Mesh：

Substances：

Year:  2007        PMID： 17578679     DOI： 10.1007/s11046-007-9033-8

Source DB:  PubMed          Journal:  Mycopathologia        ISSN： 0301-486X            Impact factor:   2.574


  13 in total

1.  Serological differences in three Blastomyces dermatitidis strains.

Authors:  R C Axtell; G M Scalarone
Journal:  Mycoses       Date:  2002-12       Impact factor: 4.377

2.  Cross-reactivity in Histoplasma capsulatum variety capsulatum antigen assays of urine samples from patients with endemic mycoses.

Authors:  J Wheat; H Wheat; P Connolly; M Kleiman; K Supparatpinyo; K Nelson; R Bradsher; A Restrepo
Journal:  Clin Infect Dis       Date:  1997-06       Impact factor: 9.079

3.  Detection of IgG and IgM in sera from canines with blastomycosis using eight blastomyces dermatitidis yeast phase lysate antigens.

Authors:  Christine M Sestero; Gene M Scalarone
Journal:  Mycopathologia       Date:  2006-07       Impact factor: 2.574

4.  Blastomyces dermatitidis antigen detection in urine specimens from dogs with blastomycosis using a competitive binding inhibition ELISA.

Authors:  J F Shurley; A M Legendre; G M Scalarone
Journal:  Mycopathologia       Date:  2005-09       Impact factor: 2.574

5.  Comparison of seven Blastomyces dermatitidis antigens for the detection of antibodies in humans with occupationally acquired blastomycosis.

Authors:  P L Roomiany; R C Axtell; G M Scalarone
Journal:  Mycoses       Date:  2002-10       Impact factor: 4.377

6.  Comparative serological evaluation of 10 Blastomyces dermatitidis yeast phase lysate antigens from different sources.

Authors:  R O Abuodeh; E M Chester; G M Scalarone
Journal:  Mycoses       Date:  2004-04       Impact factor: 4.377

7.  Sensitivity and specificity of an isoelectric focusing fraction of Blastomyces dermatitidis yeast lysate antigen for the detection of canine blastomycosis.

Authors:  M A Fisher; J L Bono; R O Abuodeh; A M Legendre; G M Scalarone
Journal:  Mycoses       Date:  1995 May-Jun       Impact factor: 4.377

8.  Detection of antibodies and delayed hypersensitivity with Rotofor preparative IEF fractions of Blastomyces dermatitidis yeast phase lysate antigen.

Authors:  J L Bono; A M Legendre; G M Scalarone
Journal:  J Med Vet Mycol       Date:  1995 Jul-Aug

9.  Preparation and ELISA evaluation of Blastomyces dermatitidis yeast phase lysate antigens.

Authors:  S M Johnson; G M Scalarone
Journal:  Diagn Microbiol Infect Dis       Date:  1988-10       Impact factor: 2.803

10.  Use of an alkali-soluble water-soluble extract of Blastomyces dermatitidis yeast-phase cell walls and isoelectrically focused components in peripheral lymphocyte transformations.

Authors:  N K Hall; F Deighton; H W Larsh
Journal:  Infect Immun       Date:  1978-02       Impact factor: 3.441
View more
  2 in total

1.  Blastomyces dermatitidis Antibody and Antigen Detection: Comparison of Four Lysate Antigens and Antibodies Prepared from Human Isolates from a Blastomycosis Outbreak.

Authors:  Jordan T Hammon; Bryn C Kennell; Gene M Scalarone
Journal:  Mycopathologia       Date:  2019-10-03       Impact factor: 2.574

2.  Blastomyces dermatitidis Yeast Lysate Antigen Combinations: Antibody Detection in Dogs with Blastomycosis.

Authors:  Alex R Boyd; Jamie L Vandyke; Gene M Scalarone
Journal:  Vet Med Int       Date:  2013-10-08
  2 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.