AIM: To further evaluate the underlying mechanism of a formerly demonstrated immature anti-inflammatory response in neonates (1). METHODS: Interleukin (IL)-10 production was measured by enzyme-linked immunosorbent-assay (ELISA) after anti-CD3/anti-CD28 costimulation of neonatal and adult T cells. IL-10 receptor expression on T lymphocytes, B lymphocytes and monocytes were analysed by flow cytometry in neonates and adult controls. RESULTS: After anti-CD3/anti-CD28 costimulation, IL-10 production of neonatal T lymphocytes was profoundly reduced (median 247 pg/mL vs. 1062 pg/mL, p < 0.0001). IL-10 receptor expression was diminished on neonatal T lymphocytes compared to adults (3% vs. 39.5% IL-10 receptor positive lymphocytes; p < 0.0001). On neonatal B lymphocytes and monocytes the IL-10 receptor expression was comparable to adult controls. CONCLUSION: The strongly reduced IL-10 receptor expression on the main immune regulative T lymphocytes in conjunction with a significantly impaired synthesis of IL-10 may play a crucial role in the formerly demonstrated deficient anti-inflammatory immune response in neonates.
AIM: To further evaluate the underlying mechanism of a formerly demonstrated immature anti-inflammatory response in neonates (1). METHODS:Interleukin (IL)-10 production was measured by enzyme-linked immunosorbent-assay (ELISA) after anti-CD3/anti-CD28 costimulation of neonatal and adult T cells. IL-10 receptor expression on T lymphocytes, B lymphocytes and monocytes were analysed by flow cytometry in neonates and adult controls. RESULTS: After anti-CD3/anti-CD28 costimulation, IL-10 production of neonatal T lymphocytes was profoundly reduced (median 247 pg/mL vs. 1062 pg/mL, p < 0.0001). IL-10 receptor expression was diminished on neonatal T lymphocytes compared to adults (3% vs. 39.5% IL-10 receptor positive lymphocytes; p < 0.0001). On neonatal B lymphocytes and monocytes the IL-10 receptor expression was comparable to adult controls. CONCLUSION: The strongly reduced IL-10 receptor expression on the main immune regulative T lymphocytes in conjunction with a significantly impaired synthesis of IL-10 may play a crucial role in the formerly demonstrated deficient anti-inflammatory immune response in neonates.
Authors: J Pagel; A Hartz; J Figge; C Gille; S Eschweiler; K Petersen; L Schreiter; J Hammer; C M Karsten; D Friedrich; E Herting; W Göpel; J Rupp; C Härtel Journal: Clin Exp Immunol Date: 2016-06-12 Impact factor: 4.330
Authors: Juliana Reis Machado; Danilo Figueiredo Soave; Marcos Vinícius da Silva; Liliana Borges de Menezes; Renata Margarida Etchebehere; Maria Luiza Gonçalves dos Reis Monteiro; Marlene Antônia dos Reis; Rosana Rosa Miranda Corrêa; Mara Rúbia Nunes Celes Journal: Mediators Inflamm Date: 2014-12-30 Impact factor: 4.711
Authors: Julia Pagel; Nele Twisselmann; Tanja K Rausch; Silvio Waschina; Annika Hartz; Magdalena Steinbeis; Jonathan Olbertz; Kathrin Nagel; Alena Steinmetz; Kirstin Faust; Martin Demmert; Wolfgang Göpel; Egbert Herting; Jan Rupp; Christoph Härtel Journal: Front Immunol Date: 2020-09-30 Impact factor: 7.561