| Literature DB >> 17577056 |
Benoit Vincart1, Ricardo De Mendonça1, Sylvianne Rottiers1, Françoise Vermeulen2, Marc J Struelens1, Olivier Denis1.
Abstract
A novel real-time PCR (RT-PCR) assay was developed for detection of Bordetella pertussis in respiratory specimens by targeting the pertactin gene. In vitro evaluation with reference strains and quality control samples showed analytical sensitivity equivalent to and specificity superior to those of PCR assays which target the IS481 element. The pertactin-based RT-PCR assay offers better discrimination between B. pertussis and other Bordetella species than previously described assays.Entities:
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Year: 2007 PMID： 17577056 DOI： 10.1099/jmm.0.46947-0
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472