OBJECT: To investigate the influence of different microenvironments on tumor microcirculation patterns and invasiveness capability. METHODS: Melanoma B16 cells were injected into the abdominal cavity and skeletal muscle of C57 mice synchronously. CK-18 expression in melanoma was assessed to distinguish the malignant phenotype of tumors in the abdominal cavity from that in the skeletal muscle. HIF-1alpha, MMP-2 and MMP-9 expression and mRNA levels of MMP-2 and MMP-9 was detected to compare the mRNA levels of MMP-2 and MMP-9 from the two microenvironments. Cells positive for each immunohistochemical stain and the vessels representative of each type of microcirculation pattern were counted in two microenvironments. RESULTS: CK-18 and HIF-1alpha expression of melanoma were significantly higher in the skeletal muscle than in the abdominal cavity (P<0.05). Compared with the abdominal cavity, melanoma cells in the skeletal muscle overexpressed MMP-2 and MMP-9 (P<0.05). Real time-PCR results also showed that MMP-2 and MMP-9 mRNA levels of melanoma were higher in the skeletal muscle than in the abdominal cavity (P<0.05). VM channels and endothelium-dependent vessels were the major microcirculation pattern in the skeletal muscle and in the abdominal cavity, respectively. CONCLUSION: Different microenvironments affect invasiveness and blood supply patterns of melanoma.
OBJECT: To investigate the influence of different microenvironments on tumor microcirculation patterns and invasiveness capability. METHODS:Melanoma B16 cells were injected into the abdominal cavity and skeletal muscle of C57 mice synchronously. CK-18 expression in melanoma was assessed to distinguish the malignant phenotype of tumors in the abdominal cavity from that in the skeletal muscle. HIF-1alpha, MMP-2 and MMP-9 expression and mRNA levels of MMP-2 and MMP-9 was detected to compare the mRNA levels of MMP-2 and MMP-9 from the two microenvironments. Cells positive for each immunohistochemical stain and the vessels representative of each type of microcirculation pattern were counted in two microenvironments. RESULTS:CK-18 and HIF-1alpha expression of melanoma were significantly higher in the skeletal muscle than in the abdominal cavity (P<0.05). Compared with the abdominal cavity, melanoma cells in the skeletal muscle overexpressed MMP-2 and MMP-9 (P<0.05). Real time-PCR results also showed that MMP-2 and MMP-9 mRNA levels of melanoma were higher in the skeletal muscle than in the abdominal cavity (P<0.05). VM channels and endothelium-dependent vessels were the major microcirculation pattern in the skeletal muscle and in the abdominal cavity, respectively. CONCLUSION: Different microenvironments affect invasiveness and blood supply patterns of melanoma.
Authors: R E Seftor; E A Seftor; N Koshikawa; P S Meltzer; L M Gardner; M Bilban; W G Stetler-Stevenson; V Quaranta; M J Hendrix Journal: Cancer Res Date: 2001-09-01 Impact factor: 12.701
Authors: Angela R Hess; Lynne-Marie Postovit; Naira V Margaryan; Elisabeth A Seftor; Galen B Schneider; Richard E B Seftor; Brian J Nickoloff; Mary J C Hendrix Journal: Cancer Res Date: 2005-11-01 Impact factor: 12.701
Authors: Einar K Rofstad; Siv H Tunheim; Berit Mathiesen; Bjørn A Graff; Ellen F Halsør; Kristin Nilsen; Kanthi Galappathi Journal: Cancer Res Date: 2002-02-01 Impact factor: 12.701
Authors: Mary J C Hendrix; Elisabeth A Seftor; Dawn A Kirschmann; Vito Quaranta; Richard E B Seftor Journal: Ann N Y Acad Sci Date: 2003-05 Impact factor: 5.691
Authors: Yue-mei Ma; Tao Sun; Yi-xin Liu; Nan Zhao; Qiang Gu; Dan-fang Zhang; Shuo Qie; Chun-sheng Ni; Yi Liu; Bao-cun Sun Journal: J Exp Clin Cancer Res Date: 2009-02-19