| Literature DB >> 17574245 |
Yoshihiro Matsumura1, Hiromichi Sakai, Mayumi Sasaki, Nobuhiro Ban, Nobuya Inagaki.
Abstract
ABCA3 is proposed to function as a lung surfactant lipid transporter. Here we report ABCA3-dependent lipid uptake into intracellular vesicles in lung adenocarcinoma A549 cells. A549 cells stably expressing GFP-tagged wild-type ABCA3 (A549/ABCA3(WT)) had larger LAMP3-positive vesicles than their parental cells as well as A549 cells expressing a Walker A motif mutant (A549/ABCA3(N568D)). The choline-phospholipids level in A549/ABCA3(WT) was increased 1.25-fold compared to that in A549 and A549/ABCA3(N568D) cells, while the cholesterol levels were similar. Sucrose gradient fractionation analysis in A549/ABCA3(WT) cells revealed that choline-phospholipids were enriched in low-density and nile red-positive vesicles. Electronmicroscopic analysis showed multilamellar vesicles in A549/ABCA3(WT) cells. These results indicate that ABCA3 mediates ATP-dependent choline-phospholipids uptake into intracellular vesicles.Entities:
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Year: 2007 PMID： 17574245 DOI： 10.1016/j.febslet.2007.05.078
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124