Literature DB >> 17565195

Expression, purification, crystallization, data collection and preliminary biochemical characterization of methicillin-resistant Staphylococcus aureus Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase.

Jaldappagari Seetharamappa1, Muse Oke, Huanting Liu, Stephen A McMahon, Kenneth A Johnson, Lester Carter, Mark Dorward, Michal Zawadzki, Ian M Overton, C A Johannes van Niekirk, Shirley Graham, Catherine H Botting, Garry L Taylor, Malcolm F White, Geoffrey J Barton, Peter J Coote, James H Naismith.   

Abstract

Sar2028, an aspartate/tyrosine/phenylalanine pyridoxal-5'-phosphate-dependent aminotransferase with a molecular weight of 48,168 Da, was overexpressed in methicillin-resistant Staphylococcus aureus compared with a methicillin-sensitive strain. The protein was expressed in Escherichia coli, purified and crystallized. The protein crystallized in a primitive orthorhombic Laue group with unit-cell parameters a = 83.6, b = 91.3, c = 106.0 A, alpha = beta = gamma = 90 degrees. Analysis of the systematic absences along the three principal axes indicated the space group to be P2(1)2(1)2(1). A complete data set was collected to 2.5 A resolution.

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Year:  2007        PMID： 17565195      PMCID： PMC2335000          DOI： 10.1107/S1744309107019562

Source DB:  PubMed          Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun        ISSN： 1744-3091


  13 in total

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Review 7.  A functional classification scheme for beta-lactamases and its correlation with molecular structure.

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  2 in total

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Authors:  Muse Oke; Lester G Carter; Kenneth A Johnson; Huanting Liu; Stephen A McMahon; Xuan Yan; Melina Kerou; Nadine D Weikart; Nadia Kadi; Md Arif Sheikh; Stefan Schmelz; Mark Dorward; Michal Zawadzki; Christopher Cozens; Helen Falconer; Helen Powers; Ian M Overton; C A Johannes van Niekerk; Xu Peng; Prakash Patel; Roger A Garrett; David Prangishvili; Catherine H Botting; Peter J Coote; David T F Dryden; Geoffrey J Barton; Ulrich Schwarz-Linek; Gregory L Challis; Garry L Taylor; Malcolm F White; James H Naismith
Journal:  J Struct Funct Genomics       Date:  2010-04-24

2.  Purification, crystallization and data collection of methicillin-resistant Staphylococcus aureus Sar2676, a pantothenate synthetase.

Authors:  Jaldappagari Seetharamappa; Muse Oke; Huanting Liu; Stephen A McMahon; Kenneth A Johnson; Lester Carter; Mark Dorward; Michal Zawadzki; Ian M Overton; C A Johannes van Niekirk; Shirley Graham; Catherine H Botting; Garry L Taylor; Malcolm F White; Geoffrey J Barton; Peter J Coote; James H Naismith
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2007-05-05
  2 in total

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