Literature DB >> 17563857

A modified Coomassie Brilliant Blue staining method at nanogram sensitivity compatible with proteomic analysis.

Xuchu Wang1, Xiaofang Li, Yinxin Li.   

Abstract

A more sensitive and convenient Coomassie Brilliant Blue (CBB) staining method for visualizing proteins was developed. Compared with the modifications include the supplement of 10% (v/v) methanol into the fixing solution, an increase of an additional sensitization step and CBB raised from 0.1 to 0.125%. The improved method can detect proteins at nanogram level. The improved method is more sensitive than Blue Silver and more convenient than the Silver protocol. Mass spectrometry results confirmed that it is suitable for subsequent proteomic research.

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Year:  2007        PMID： 17563857     DOI： 10.1007/s10529-007-9425-3

Source DB:  PubMed          Journal:  Biotechnol Lett        ISSN： 0141-5492            Impact factor:   2.461


  22 in total

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Journal:  Front Plant Sci       Date:  2021-05-10       Impact factor: 5.753

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