Multinuclear magnetic resonance studies on the chemical interaction of a self-etching adhesive with radicular and coronal human dentin.

This study presents evidence at molecular level for the chemical interaction between human dentin from different tooth regions and a monomer with phosphate groups, incorporated in the formulation of a simplified adhesive system. Because dentin was observed as a powder, previous verification was obtained for an eventual collagen denaturation due to the grinding process. The presence of chemical bonds involving coronal (CD) or radicular dentin (RD) was investigated using multinuclear magnetic resonance (MR) techniques. Narrow signals were identified in the carbon magic angle spinning (MAS) spectra of CD and RD treated with the adhesive, which were assigned to methylenic groups in methacryloyloxydecyl dihydrogen phosphate (MDP) bound to hydroxyapatite Ca2+; 1H spectra of the adhesive components and treated dentin, in ethanol, support this conclusion. (31)P MAS spectra obtained from both dentin regions present additional shielding and broadening effects subsequent to application and photopolymerization of the adhesive, which were higher for CD. Multinuclear MR studies provided evidence for the interaction of the adhesive with dentin, which involves hydroxyapatite and is stronger for CD than for RD, but no direct proof was obtained on bonding to collagen.