Literature DB >> 17561452

Measurement of amino acid isotope enrichment by liquid chromatography mass spectroscopy after derivatization with 9-fluorenylmethylchloroformate.

Hans M H van Eijk1, Dennis P L Suylen, Cornelis H C Dejong, Yvette C Luiking, Nicolaas E P Deutz.   

Abstract

An automated method is described to measure tracer-tracee-ratios (TTR) of plasma amino acids after their separation as 9-fluorenylmethylchloroformate derivatives. In a 45 min cycle, 5 microl plasma aliquots were derivatized, HPLC separated and subjected to electrospray ionization. By applying source collision induced dissociation, derivatives were dissociated at the peptide bond releasing the originating amino acids into the mass spectrometer. This approach enabled the determination of plasma amino acid TTRs with a standard deviation between 0.15 and 0.36%, which is sufficient to study the fate of infused tracers and their conversion products in an in vivo experiment in humans.

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Year:  2007        PMID： 17561452     DOI： 10.1016/j.jchromb.2007.05.020

Source DB:  PubMed          Journal:  J Chromatogr B Analyt Technol Biomed Life Sci        ISSN： 1570-0232            Impact factor:   3.205


  8 in total

1.  Nitric oxide and L-arginine metabolism in a devascularized porcine model of acute liver failure.

Authors:  Vikram Sharma; Gabriella A M Ten Have; Lars Ytrebo; Sambit Sen; Christopher F Rose; R Neil Dalton; Charles Turner; Arthur Revhaug; Hans M H van-Eijk; Nicolaas E P Deutz; Rajiv Jalan; Rajeshwar P Mookerjee; Nathan A Davies
Journal:  Am J Physiol Gastrointest Liver Physiol       Date:  2012-03-15       Impact factor: 4.052

2.  Stimulated nitric oxide production and arginine deficiency in children with cystic fibrosis with nutritional failure.

Authors:  Mariëlle P K J Engelen; Gulnur Com; Yvette C Luiking; Nicolaas E P Deutz
Journal:  J Pediatr       Date:  2013-02-15       Impact factor: 4.406

3.  Hydrolyzed casein and whey protein meals comparably stimulate net whole-body protein synthesis in COPD patients with nutritional depletion without an additional effect of leucine co-ingestion.

Authors:  Renate Jonker; Nicolaas E P Deutz; Marcia L Erbland; Paula J Anderson; Mariëlle P K J Engelen
Journal:  Clin Nutr       Date:  2013-07-01       Impact factor: 7.324

4.  Essential amino acid-enriched meal replacement promotes superior net protein balance in older, overweight adults.

Authors:  Robert H Coker; Kenneth Shin; Kristen Scholten; Michelle Johannsen; Jean Tsigonis; Il-Young Kim; Scott E Schutzler; Robert R Wolfe
Journal:  Clin Nutr       Date:  2018-12-19       Impact factor: 7.324

5.  Enhanced anabolic response to milk protein sip feeding in elderly subjects with COPD is associated with a reduced splanchnic extraction of multiple amino acids.

Authors:  M P K J Engelen; C L N De Castro; E P A Rutten; E F M Wouters; A M W J Schols; N E P Deutz
Journal:  Clin Nutr       Date:  2012-06-06       Impact factor: 7.324

6.  Casein protein results in higher prandial and exercise induced whole body protein anabolism than whey protein in chronic obstructive pulmonary disease.

Authors:  Mariëlle P K J Engelen; Erica P A Rutten; Carmen L N De Castro; Emiel F M Wouters; Annemie M W J Schols; Nicolaas E P Deutz
Journal:  Metabolism       Date:  2012-04-17       Impact factor: 8.694

7.  Increased protein-energy intake promotes anabolism in critically ill infants with viral bronchiolitis: a double-blind randomised controlled trial.

Authors:  Carlijn T de Betue; Dick A van Waardenburg; Nicolaas E Deutz; Hans M van Eijk; Johannes B van Goudoever; Yvette C Luiking; Luc J Zimmermann; Koen F Joosten
Journal:  Arch Dis Child       Date:  2011-06-14       Impact factor: 3.791

8.  Features, causes and consequences of splanchnic sequestration of amino acid in old rats.

Authors:  Marion Jourdan; Nicolaas E P Deutz; Luc Cynober; Christian Aussel
Journal:  PLoS One       Date:  2011-11-08       Impact factor: 3.240
  8 in total

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