| Literature DB >> 17561372 |
Rong Liang1, Wei-Dong Yu, Jun-Bao Du, Li-Jun Yang, Jing-Jing Yang, Jian Xu, Mei Shang, Jing-Zhu Guo.
Abstract
Our previous study demonstrated that cystathionine beta synthase (CBS) is highly expressed in the cumulus-oocyte complex during ovulation. However, the role of CBS during oocyte maturation remains uncertain. In this study, a small-interfering (si) RNA interference (siRNA) approach was used to investigate the potential role of CBS during oocyte maturation. Accompanied with a gradual increase of homocysteine, the introduction of CBS-siRNA into murine granulosa cells selectively depleted the corresponding target mRNA and protein for CBS as assessed by semi-quantitative reverse-transcriptase PCR (RT-PCR) and immunofluorescence staining. When fully grown, germinal vesicle (GV) oocytes matured in vitro for 16 h using medium from transfected granulosa cells, the functional suppression of CBS resulted in a significant increase in the rate of GV-arrested oocytes. The results of this study provide evidence that CBS participates in the process of oocyte maturation. Furthermore, this effect may be fulfilled by conditioning the level of homocysteine in the microenvironment of the oocyte.Entities:
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Year: 2007 PMID： 17561372 DOI： 10.1016/j.reprotox.2007.04.002
Source DB: PubMed Journal: Reprod Toxicol ISSN： 0890-6238 Impact factor: 3.143