| Literature DB >> 17560798 |
David Lin1, Joseph Najbauer, Paul M Salvaterra, Adam N Mamelak, Michael E Barish, Elizabeth Garcia, Marianne Z Metz, Stephen E Kendall, Marisa Bowers, Babak Kateb, Seung U Kim, Margaret Johnson, Karen S Aboody.
Abstract
Neural stem cells (NSCs) hold great promise for glioma therapy due to their inherent tumor-tropic properties, enabling them to deliver therapeutic agents directly to invasive tumor sites. In the present study, we visualized and quantitatively analyzed the spatial distribution of tumor-tropic NSCs in a mouse model of orthotopic glioma in order to predict the therapeutic efficacy of a representative NSC-based glioma therapy. U251.eGFP human glioma was established in the brain of athymic mice, followed by stereotactic injection of CM-DiI-labeled human NSCs posterior-lateral to the tumor site. Confocal microscopy, three-dimensional modeling and mathematical algorithms were used to visualize and characterize the spatial distribution of NSCs throughout the tumor. The pattern of NSC distribution showed a gradient with higher densities toward the centroid of the tumor mass. We estimate that NSC-mediated therapy would eradicate 70-90% of the primary tumor mass and the majority of invasive tumor foci. Our method may serve as a model for optimizing the efficacy of NSC-based glioma therapy.Entities:
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Year: 2007 PMID： 17560798 DOI： 10.1016/j.neuroimage.2007.03.076
Source DB: PubMed Journal: Neuroimage ISSN： 1053-8119 Impact factor: 6.556