Effects of CpG-oligonucleotides, poly I:C and LPS on Atlantic cod scavenger endothelial cells (SEC).

Carrying out a remarkably efficient clearance of colloidal and soluble macromolecular waste substances from the circulation, the scavenger endothelial cells (SECs) represent an important part of the reticuloendothelial system of vertebrates. It has been previously shown that these cells play an important role in the innate immune system by eliminating from the blood a number of molecules known to elicit inflammatory reactions. In the present study we have investigated the uptake of LPS and oligonucleotides in cultured Atlantic cod SECs, and determined if interaction with these pathogen associated molecules affect the scavenger activity and/or production of immune modulating molecules of SECs. Preincubation of cultured SECs with CpG (5 and 20 microg/ml) or Poly I:C (10 or 40 microg/ml) gave selective down-regulation of scavenger receptor-mediated endocytosis, but only marginal effects were noted on endocytosis via the mannose- and collagen alpha chain receptors. Preincubation with LPS or a non-inflammatory ligand for the scavenger receptor did not result in altered endocytosis via any of the receptors tested. Only Poly I:C (40 microg/ml) was observed to increase the production of NO. RT-PCR analyses showed IL-1 production which was not increased above control after pre-treatment with two different CpG-oligonucleotides or Poly I:C. In fact, preincubation with Poly I:C, but not CpGs, resulted in degradation of total intracellular RNA. In conclusion, our study shows that SECs respond differently to the different immunomodulators used and that their important clearance activity as scavenger cells can be regulated by the use of oligonucleotides.