BACKGROUND: IL-13 is a key cytokine associated with the asthmatic phenotype. IL-13 signals via its cognate receptor, a complex of IL-13 receptor (IL-13R) alpha 1 chain with IL-4 receptor alpha; however, a second protein, IL-13Ralpha2, also binds IL-13. Recently a polymorphic variant of IL-13 (R110Q) has been shown to be associated with atopy. OBJECTIVE: To investigate the binding properties of this IL-13 variant to its cognate receptors. METHODS: We used surface plasmon resonance to measure the binding kinetics of R110Q to its receptors. Primary human fibroblasts were grown from endobronchial biopsies obtained from volunteers. Receptor levels were measured by fluorescence-activated cell sorting. RESULTS: There was no significant difference in the binding of R110Q with soluble human IL-13Ralpha1 compared with IL-13 (32 +/- 5 nmol/L and 36 +/- 7 nmol/L, respectively; P = .625). However, a small but significant difference was observed in the binding of R110Q to soluble human IL-13Ralpha2 compared with IL-13 (840 +/- 87 pmol/L and 1.1 +/- .05 nmol/L, respectively; P = .04). We observed that primary human lung fibroblasts expressed different levels of IL-13Ralpha2. Eotaxin release from fibroblasts expressing low IL-13Ralpha2 levels was significantly higher in response to R110Q compared with IL-13. This was not evident in cells that had high baseline IL-13Ralpha2 levels. CONCLUSION: These results suggest that relatively small changes in functional properties of a ligand combined with variation in receptor levels in vivo can result in significant differences in responsiveness. CLINICAL IMPLICATIONS: Expression of R110Q and low IL-13Ralpha2 levels can result in important biological differences that may have clinical relevance in an atopic environment.
BACKGROUND: IL-13 is a key cytokine associated with the asthmatic phenotype. IL-13 signals via its cognate receptor, a complex of IL-13 receptor (IL-13R) alpha 1 chain with IL-4 receptor alpha; however, a second protein, IL-13Ralpha2, also binds IL-13. Recently a polymorphic variant of IL-13 (R110Q) has been shown to be associated with atopy. OBJECTIVE: To investigate the binding properties of this IL-13 variant to its cognate receptors. METHODS: We used surface plasmon resonance to measure the binding kinetics of R110Q to its receptors. Primary human fibroblasts were grown from endobronchial biopsies obtained from volunteers. Receptor levels were measured by fluorescence-activated cell sorting. RESULTS: There was no significant difference in the binding of R110Q with soluble human IL-13Ralpha1 compared with IL-13 (32 +/- 5 nmol/L and 36 +/- 7 nmol/L, respectively; P = .625). However, a small but significant difference was observed in the binding of R110Q to soluble human IL-13Ralpha2 compared with IL-13 (840 +/- 87 pmol/L and 1.1 +/- .05 nmol/L, respectively; P = .04). We observed that primary human lung fibroblasts expressed different levels of IL-13Ralpha2. Eotaxin release from fibroblasts expressing low IL-13Ralpha2 levels was significantly higher in response to R110Q compared with IL-13. This was not evident in cells that had high baseline IL-13Ralpha2 levels. CONCLUSION: These results suggest that relatively small changes in functional properties of a ligand combined with variation in receptor levels in vivo can result in significant differences in responsiveness. CLINICAL IMPLICATIONS: Expression of R110Q and low IL-13Ralpha2 levels can result in important biological differences that may have clinical relevance in an atopic environment.
Authors: Sayantan Das; Philip E Stuart; Jun Ding; Trilokraj Tejasvi; Yanming Li; Lam C Tsoi; Vinod Chandran; Judith Fischer; Cynthia Helms; Kristina Callis Duffin; John J Voorhees; Anne M Bowcock; Gerald G Krueger; G Mark Lathrop; Rajan P Nair; Proton Rahman; Goncalo R Abecasis; Dafna Gladman; James T Elder Journal: Eur J Hum Genet Date: 2014-09-03 Impact factor: 4.246
Authors: Yujing Du; Zhao Chen; Xiaojiang Duan; Ping Yan; Chunli Zhang; Lei Kang; Rongfu Wang Journal: Ann Nucl Med Date: 2022-01-15 Impact factor: 2.668
Authors: Ali H Ziyab; Gwyneth A Davies; Susan Ewart; Julian M Hopkin; Eric M Schauberger; Marsha Wills-Karp; John W Holloway; Syed Hasan Arshad; Hongmei Zhang; Wilfried Karmaus Journal: BMC Med Genet Date: 2013-07-02 Impact factor: 2.103