BACKGROUND: Recently, a new polymorphic gene family called the major histocompatibility complex class I chain-related gene A (MICA) was discovered about 40 kb centromeric to HLA-B gene. The MICA protein, expressed on epithelial cells and many kinds of tumor cells, serves to regulate immune function. The MICA protein is thought to activate immune function on mucosal tissue by binding to NKG2D which is expressed on most natural killer cells, CD8 positive T cells, and gamma delta T cells. An association between MICA gene polymorphisms and the development of oral squamous cell carcinoma (OSCC) has also been reported. OBJECTIVE: This study was designed to test this association in Japanese patients with OSCC. METHODS: The (GCT)(n) polymorphisms of the MICA gene was investigated in 123 patients with OSCC and 188 normal controls using polymerase chain reaction amplification and denaturing polyacrylamide gel electrophoresis. RESULTS: Five alleles, namely A4, A5, A6, A9, and A5.1, were found in both groups. The phenotype frequency of the MICA-A5.1 allele was significantly higher in patients with OSCC when compared with normal controls (OR 1.707, 95% CI 0.76-3.45, P=0.042). Also, the microsatellite frequency of the MICA-A5.1 allele was significantly higher in patients with OSCC compared with normal controls (OR 1.664, 95% CI 0.82-3.42, P=0.021). Lastly, the frequency of the MICA-A5.1 allele was significantly higher in those with lymph node metastasis from OSCC compared with normal controls (OR 2.605, 95% CI 1.14-5.27, P=0.026). CONCLUSIONS: These results suggest that the MICA-A5.1 allele may be associated with an increased susceptibility to OSCC in Japan.
BACKGROUND: Recently, a new polymorphic gene family called the major histocompatibility complex class I chain-related gene A (MICA) was discovered about 40 kb centromeric to HLA-B gene. The MICA protein, expressed on epithelial cells and many kinds of tumor cells, serves to regulate immune function. The MICA protein is thought to activate immune function on mucosal tissue by binding to NKG2D which is expressed on most natural killer cells, CD8 positive T cells, and gamma delta T cells. An association between MICA gene polymorphisms and the development of oral squamous cell carcinoma (OSCC) has also been reported. OBJECTIVE: This study was designed to test this association in Japanese patients with OSCC. METHODS: The (GCT)(n) polymorphisms of the MICA gene was investigated in 123 patients with OSCC and 188 normal controls using polymerase chain reaction amplification and denaturing polyacrylamide gel electrophoresis. RESULTS: Five alleles, namely A4, A5, A6, A9, and A5.1, were found in both groups. The phenotype frequency of the MICA-A5.1 allele was significantly higher in patients with OSCC when compared with normal controls (OR 1.707, 95% CI 0.76-3.45, P=0.042). Also, the microsatellite frequency of the MICA-A5.1 allele was significantly higher in patients with OSCC compared with normal controls (OR 1.664, 95% CI 0.82-3.42, P=0.021). Lastly, the frequency of the MICA-A5.1 allele was significantly higher in those with lymph node metastasis from OSCC compared with normal controls (OR 2.605, 95% CI 1.14-5.27, P=0.026). CONCLUSIONS: These results suggest that the MICA-A5.1 allele may be associated with an increased susceptibility to OSCC in Japan.
Authors: Hongliang Liu; Jian Wang; Robert Yu; Sanjay Shete; Erich M Sturgis; Guojun Li; Kristina R Dahlstrom; Zhensheng Liu; Christopher I Amos; Qingyi Wei Journal: Cancer Res Date: 2020-04-10 Impact factor: 12.701
Authors: Guillaume Onyeaghala; John Lane; Nathan Pankratz; Heather H Nelson; Bharat Thyagarajan; Bruce Walcheck; Kristin E Anderson; Anna E Prizment Journal: PLoS One Date: 2019-06-05 Impact factor: 3.240
Authors: Karen Toledo-Stuardo; Carolina H Ribeiro; Andrea Canals; Marcela Morales; Valentina Gárate; Jose Rodríguez-Siza; Samantha Tello; Marco Bustamante; Ricardo Armisen; Douglas J Matthies; Gerald Zapata-Torres; Patricio González-Hormazabal; María Carmen Molina Journal: Front Immunol Date: 2021-03-31 Impact factor: 7.561