BACKGROUND: Although it is well known that TGF-beta circulates, the presence and activity of endogenous bone morphogenetic proteins (BMPs) in biological fluids has not been studied. Here we investigated the urine secretion of a systemically administered BMP hybrid molecule. METHODS: A dimeric recombinant human BMP molecule consisting of the BMP-7 prodomain and the BMP-6 mature domain was constructed and injected into Sprague Dawley rats. The blood was collected from the rats' orbital plexus, and 24-hour urine samples were pooled and purified using a heparin sepharose column. Protein identity was confirmed by Western blot and by liquid chromatography-mass spectrometry (LC-MS) of the resulting peptides. Urine-derived protein from the 35-kDa band was bound to inactive demineralized bone matrix and implanted subcutaneously into rats. RESULTS: Western blot analysis of sera demonstrated that BMP-7/6 remained intact in the rat plasma and could still be visualized 30 minutes after its systemic administration. Two protein bands at 35 and 39 kDa were detected with anti-BMP antibodies in the urine of rats, corresponding to the mature active BMP-6 dimer and the prodomain of BMP-7, respectively. LC-MS analysis detected only peptides derived from the BMP-7/6 molecule. Histological analysis of implanted pellets revealed formation of a new endochondral bone 14 days following implantation. CONCLUSIONS: These results show for the first time that systemically administered BMP-7/6 hybrid molecule is secreted into the urine and that its biological activity is preserved, suggesting that analysis of BMP in urine might reflect its presence in serum.
BACKGROUND: Although it is well known that TGF-beta circulates, the presence and activity of endogenous bone morphogenetic proteins (BMPs) in biological fluids has not been studied. Here we investigated the urine secretion of a systemically administered BMP hybrid molecule. METHODS: A dimeric recombinant humanBMP molecule consisting of the BMP-7 prodomain and the BMP-6 mature domain was constructed and injected into Sprague Dawley rats. The blood was collected from the rats' orbital plexus, and 24-hour urine samples were pooled and purified using a heparinsepharose column. Protein identity was confirmed by Western blot and by liquid chromatography-mass spectrometry (LC-MS) of the resulting peptides. Urine-derived protein from the 35-kDa band was bound to inactive demineralized bone matrix and implanted subcutaneously into rats. RESULTS: Western blot analysis of sera demonstrated that BMP-7/6 remained intact in the rat plasma and could still be visualized 30 minutes after its systemic administration. Two protein bands at 35 and 39 kDa were detected with anti-BMP antibodies in the urine of rats, corresponding to the mature active BMP-6 dimer and the prodomain of BMP-7, respectively. LC-MS analysis detected only peptides derived from the BMP-7/6 molecule. Histological analysis of implanted pellets revealed formation of a new endochondral bone 14 days following implantation. CONCLUSIONS: These results show for the first time that systemically administered BMP-7/6 hybrid molecule is secreted into the urine and that its biological activity is preserved, suggesting that analysis of BMP in urine might reflect its presence in serum.
Authors: Lovorka Grgurevic; Boris Macek; David R Healy; Amy L Brault; Igor Erjavec; Antonio Cipcic; Ivica Grgurevic; Dunja Rogic; Kresimir Galesic; Jelena Brkljacic; Ranka Stern-Padovan; Vishwas M Paralkar; Slobodan Vukicevic Journal: J Am Soc Nephrol Date: 2011-03-17 Impact factor: 10.121