Developmental pattern changes of prefrontal efferents in the juvenile gerbil (Meriones unguiculatus).

Previous findings of our group showed that early traumatisation leads to a dysfunctional organisation of prefrontocortical efferents in adulthood. To identify vulnerable time windows during maturation, we labelled either layer III- or layer V/VI-pyramidal cells with biocytin in the prefrontal cortex of gerbils (Meriones unguiculatus) from the age of postnatal day (PD) 15 up to adulthood (PD 90). The density of passing fibres and axonal terminals in distinct cortical columns in specific prefrontal projection areas was assessed by digital image analysis. Following layer III injections, fibre densities reached adult values between adolescence (PD 60) and adulthood (PD 90). However, layer V/VI-fibre densities decreased after eye-opening (PD 15), followed by an increase to adult values after weaning (PD 30). These findings are the first to describe dynamic structural changes even beyond adolescence of functionally diverse prefrontal output systems. External interventions might exert adverse influences on the establishment of integrated prefrontal networks especially during the early phase of re-arranging.