OBJECTIVE: To prepare the monoclonal antibody (McAb) against citrinin. METHODS: Four citrinin-protein conjugates (A, B, C and D) were synthesized by the active ester, Munnich Reaction and 1,1-carbonyldiimidazole (CDI) method, respectively. BALB/c mice were immunized with the conjugates for producing the McAb against citrinin. RESULTS: The antibodies against citrinin have been produced in serum of immunized BALB/c mice with the conjugate C. A McAb against citrinin was acquired according to the traditional procedure. Cross reactivity of the McAb was less than 0.01% with ochratoxin A, patulin, aflatoxin B1, deoxynivalenol and zearalenone, respectively. A competitive enzyme-linked immunosorbent assay was developed. The linear range of ELISA was between 20 ng/ml and 1 000 ng/ml, the detectable limit was 10 ng/ml. Recovery of citrinin from wheat spiked with citrinin was from 95% to 112%, the coefficient of variation was 12.2% to 20.4%. CONCLUSION: The McAb against citrinin was acquired successfully.
OBJECTIVE: To prepare the monoclonal antibody (McAb) against citrinin. METHODS: Four citrinin-protein conjugates (A, B, C and D) were synthesized by the active ester, Munnich Reaction and 1,1-carbonyldiimidazole (CDI) method, respectively. BALB/c mice were immunized with the conjugates for producing the McAb against citrinin. RESULTS: The antibodies against citrinin have been produced in serum of immunized BALB/c mice with the conjugate C. A McAb against citrinin was acquired according to the traditional procedure. Cross reactivity of the McAb was less than 0.01% with ochratoxin A, patulin, aflatoxin B1, deoxynivalenol and zearalenone, respectively. A competitive enzyme-linked immunosorbent assay was developed. The linear range of ELISA was between 20 ng/ml and 1 000 ng/ml, the detectable limit was 10 ng/ml. Recovery of citrinin from wheat spiked with citrinin was from 95% to 112%, the coefficient of variation was 12.2% to 20.4%. CONCLUSION: The McAb against citrinin was acquired successfully.