OBJECTIVE: To determine the relative frequencies of reinfection vs. reactivation or treatment failure in patients from a high tuberculosis incidence setting with a low prevalence of HIV infection. METHOD: We performed DNA fingerprinting on serial isolates from one and multiple TB episodes from 97 retreatment patients; 35 patients had been previously cured, whereas 62 had not. RESULTS: DNA fingerprinting patterns of recurrence Mycobacterium tuberculosis isolates of 5 of the 35 previously cured patients did not match with those of the corresponding initial isolates, indicating reinfection. We did not document reinfection during treatment. Isolates from each of the remaining 30 previously cured patients had identical DNA fingerprinting results, indicating reactivation. DNA fingerprinting patterns of isolates from the 62 patients with persistently positive sputum smears were identical, suggesting treatment failure. CONCLUSION: These findings suggest that reinfection is not a common cause of relapse and treatment failure in this rural predominantly HIV-free population despite the high incidence of TB.
OBJECTIVE: To determine the relative frequencies of reinfection vs. reactivation or treatment failure in patients from a high tuberculosis incidence setting with a low prevalence of HIV infection. METHOD: We performed DNA fingerprinting on serial isolates from one and multiple TB episodes from 97 retreatment patients; 35 patients had been previously cured, whereas 62 had not. RESULTS: DNA fingerprinting patterns of recurrence Mycobacterium tuberculosis isolates of 5 of the 35 previously cured patients did not match with those of the corresponding initial isolates, indicating reinfection. We did not document reinfection during treatment. Isolates from each of the remaining 30 previously cured patients had identical DNA fingerprinting results, indicating reactivation. DNA fingerprinting patterns of isolates from the 62 patients with persistently positive sputum smears were identical, suggesting treatment failure. CONCLUSION: These findings suggest that reinfection is not a common cause of relapse and treatment failure in this rural predominantly HIV-free population despite the high incidence of TB.
Authors: M Gabriela M Gomes; Ricardo Aguas; João S Lopes; Marta C Nunes; Carlota Rebelo; Paula Rodrigues; Claudio J Struchiner Journal: Proc Biol Sci Date: 2012-02-22 Impact factor: 5.349
Authors: Isdore Chola Shamputa; Jongseok Lee; Caroline Allix-Béguec; Eun-Jin Cho; Ji-im Lee; Vignesh Rajan; Eun Gae Lee; Jin Hong Min; Matthew W Carroll; Lisa C Goldfeder; Jin Hee Kim; Hyung Seok Kang; Soohee Hwang; Seok-Yong Eum; Seung Kyu Park; Hyeyoung Lee; Philip Supply; Sang-Nae Cho; Laura E Via; Clifton E Barry Journal: J Clin Microbiol Date: 2009-12-16 Impact factor: 5.948