Literature DB >> 17546005

Tandem affinity purification of functional TAP-tagged proteins from human cells.

Juraj Gregan1, Christian G Riedel, Mark Petronczki, Lubos Cipak, Cornelia Rumpf, Ina Poser, Frank Buchholz, Karl Mechtler, Kim Nasmyth.   

Abstract

Tandem affinity purification (TAP) is a generic two-step affinity purification protocol for isolation of TAP-tagged proteins together with associated proteins. We used bacterial artificial chromosome to heterologously express TAP-tagged murine Sgo1 protein in human HeLa cells. This allowed us to test the functionality of the Sgo1-TAP protein by RNA interference-mediated depletion of the endogenous human Sgo1. Here, we present an optimized protocol for purification of TAP-tagged Sgo1 protein as well as KIAA1387 from HeLa cells with detailed instructions. The purification protocol can be completed in 1 day and it should be applicable to other proteins.

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Year:  2007        PMID： 17546005      PMCID： PMC2957861          DOI： 10.1038/nprot.2007.172

Source DB:  PubMed          Journal:  Nat Protoc        ISSN： 1750-2799            Impact factor:   13.491


  23 in total

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2.  Empirical statistical model to estimate the accuracy of peptide identifications made by MS/MS and database search.

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Journal:  Anal Chem       Date:  2002-10-15       Impact factor: 6.986

3.  A statistical model for identifying proteins by tandem mass spectrometry.

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4.  An efficient tandem affinity purification procedure for interaction proteomics in mammalian cells.

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Journal:  Nat Methods       Date:  2006-10-22       Impact factor: 28.547

5.  Tricine-SDS-PAGE.

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Journal:  Nat Protoc       Date:  2006       Impact factor: 13.491

6.  Functional organization of the yeast proteome by systematic analysis of protein complexes.

Authors:  Anne-Claude Gavin; Markus Bösche; Roland Krause; Paola Grandi; Martina Marzioch; Andreas Bauer; Jörg Schultz; Jens M Rick; Anne-Marie Michon; Cristina-Maria Cruciat; Marita Remor; Christian Höfert; Malgorzata Schelder; Miro Brajenovic; Heinz Ruffner; Alejandro Merino; Karin Klein; Manuela Hudak; David Dickson; Tatjana Rudi; Volker Gnau; Angela Bauch; Sonja Bastuck; Bettina Huhse; Christina Leutwein; Marie-Anne Heurtier; Richard R Copley; Angela Edelmann; Erich Querfurth; Vladimir Rybin; Gerard Drewes; Manfred Raida; Tewis Bouwmeester; Peer Bork; Bertrand Seraphin; Bernhard Kuster; Gitte Neubauer; Giulio Superti-Furga
Journal:  Nature       Date:  2002-01-10       Impact factor: 49.962

7.  New partners of acyl carrier protein detected in Escherichia coli by tandem affinity purification.

Authors:  D Gully; D Moinier; L Loiseau; E Bouveret
Journal:  FEBS Lett       Date:  2003-07-31       Impact factor: 4.124

8.  Improved tandem affinity purification tag and methods for isolation of protein heterocomplexes from plants.

Authors:  Jai S Rohila; Mei Chen; Ronald Cerny; Michael E Fromm
Journal:  Plant J       Date:  2004-04       Impact factor: 6.417

9.  A Drosophila fragile X protein interacts with components of RNAi and ribosomal proteins.

Authors:  Akira Ishizuka; Mikiko C Siomi; Haruhiko Siomi
Journal:  Genes Dev       Date:  2002-10-01       Impact factor: 11.361

10.  A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway.

Authors:  Tewis Bouwmeester; Angela Bauch; Heinz Ruffner; Pierre-Olivier Angrand; Giovanna Bergamini; Karen Croughton; Cristina Cruciat; Dirk Eberhard; Julien Gagneur; Sonja Ghidelli; Carsten Hopf; Bettina Huhse; Raffaella Mangano; Anne-Marie Michon; Markus Schirle; Judith Schlegl; Markus Schwab; Martin A Stein; Andreas Bauer; Georg Casari; Gerard Drewes; Anne-Claude Gavin; David B Jackson; Gerard Joberty; Gitte Neubauer; Jens Rick; Bernhard Kuster; Giulio Superti-Furga
Journal:  Nat Cell Biol       Date:  2004-01-25       Impact factor: 28.824
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  20 in total

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Journal:  Nat Protoc       Date:  2010-04-15       Impact factor: 13.491

2.  Online nanoflow RP-RP-MS reveals dynamics of multicomponent Ku complex in response to DNA damage.

Authors:  Feng Zhou; Job D Cardoza; Scott B Ficarro; Guillaume O Adelmant; Jean-Bernard Lazaro; Jarrod A Marto
Journal:  J Proteome Res       Date:  2010-10-27       Impact factor: 4.466

3.  Separating the wheat from the chaff: unbiased filtering of background tandem mass spectra improves protein identification.

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4.  Construction of a dual affinity tagged allele of the Rb1 tumor suppressor gene in the mouse.

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Journal:  Genesis       Date:  2010-02       Impact factor: 2.487

5.  Casein kinase 1 is required for efficient removal of Rec8 during meiosis I.

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6.  The Galaxy Platform for Reproducible Affinity Proteomic Mass Spectrometry Data Analysis.

Authors:  Paul A Stewart; Brent M Kuenzi; Subina Mehta; Praveen Kumar; James E Johnson; Pratik Jagtap; Timothy J Griffin; Eric B Haura
Journal:  Methods Mol Biol       Date:  2019

7.  High-throughput knockout screen in Schizosaccharomyces pombe identifies a novel gene required for efficient homolog disjunction during meiosis I.

Authors:  Cornelia Rumpf; Lubos Cipak; Maria Novatchkova; Zhang Li; Silvia Polakova; Andrej Dudas; Ines Kovacikova; Eva Miadokova; Gustav Ammerer; Juraj Gregan
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8.  GABAB receptor constituents revealed by tandem affinity purification from transgenic mice.

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Journal:  J Biol Chem       Date:  2010-04-20       Impact factor: 5.157

9.  Proteome-wide identification of novel binding partners to the oncogenic fusion gene protein, NPM-ALK, using tandem affinity purification and mass spectrometry.

Authors:  Fang Wu; Peng Wang; Leah C Young; Raymond Lai; Liang Li
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10.  Chromatin Central: towards the comparative proteome by accurate mapping of the yeast proteomic environment.

Authors:  Anna Shevchenko; Assen Roguev; Daniel Schaft; Luke Buchanan; Bianca Habermann; Cagri Sakalar; Henrik Thomas; Nevan J Krogan; Andrej Shevchenko; A Francis Stewart
Journal:  Genome Biol       Date:  2008-11-28       Impact factor: 13.583
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