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Literature DB >> 17545967

Protocols for growth and development of Dictyostelium discoideum.

Petra Fey¹, Anthony S Kowal, Pascale Gaudet, Karen E Pilcher, Rex L Chisholm.

Abstract

Dictyostelium discoideum, a unicellular organism capable of developing into a multicellular structure, is a powerful model system to study a variety of biological processes. Because it is inexpensive and relatively easy to grow, Dictyostelium is also frequently used in teaching laboratories. Here we describe conditions for successfully growing and developing Dictyostelium cells and methods for long-term storage of Dictyostelium amoebae and spores.

Entities: Species

Mesh：

Year: 2007 PMID： 17545967 DOI： 10.1038/nprot.2007.178

Source DB: PubMed Journal: Nat Protoc ISSN： 1750-2799 Impact factor: 13.491

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Cited

96 in total

1. Dictyostelium puromycin-sensitive aminopeptidase A is a nucleoplasmic nucleomorphin-binding protein that relocates to the cytoplasm during mitosis.

Authors: Andrew Catalano; Yekaterina Poloz; Danton H O'Day
Journal: Histochem Cell Biol Date: 2011-10-25 Impact factor: 4.304

2. Local motion analysis reveals impact of the dynamic cytoskeleton on intracellular subdiffusion.

Authors: Marcus Otten; Amitabha Nandi; Delphine Arcizet; Mari Gorelashvili; Benjamin Lindner; Doris Heinrich
Journal: Biophys J Date: 2012-02-21 Impact factor: 4.033

3. Non-invasive LC-PolScope imaging of biominerals and cell wall anisotropy changes.

Authors: Magdalena Eder; Ursula Lütz-Meindl; Ingrid M Weiss
Journal: Protoplasma Date: 2010-03-17 Impact factor: 3.356

4. Formation of Hirano bodies after inducible expression of a modified form of an actin-cross-linking protein.

Authors: Juan F Reyes; Karen Stone; Jeanie Ramos; Andrew Maselli
Journal: Eukaryot Cell Date: 2009-04-10

5. Nucleolar localization and identification of nuclear/nucleolar localization signals of the calmodulin-binding protein nucleomorphin during growth and mitosis in Dictyostelium.

Authors: Andrew Catalano; Danton H O'Day
Journal: Histochem Cell Biol Date: 2011-02-17 Impact factor: 4.304

Protocols for growth and development of Dictyostelium discoideum.

1. Dictyostelium puromycin-sensitive aminopeptidase A is a nucleoplasmic nucleomorphin-binding protein that relocates to the cytoplasm during mitosis.

2. Local motion analysis reveals impact of the dynamic cytoskeleton on intracellular subdiffusion.

3. Non-invasive LC-PolScope imaging of biominerals and cell wall anisotropy changes.

4. Formation of Hirano bodies after inducible expression of a modified form of an actin-cross-linking protein.

5. Nucleolar localization and identification of nuclear/nucleolar localization signals of the calmodulin-binding protein nucleomorphin during growth and mitosis in Dictyostelium.

6. Actin cytoskeleton of chemotactic amoebae operates close to the onset of oscillations.

7. Aberrant adhesion impacts early development in a Dictyostelium model for juvenile neuronal ceroid lipofuscinosis.

8. Transcriptional profiling of Dictyostelium with RNA sequencing.

9. α-catenin and IQGAP regulate myosin localization to control epithelial tube morphogenesis in Dictyostelium.

10. Live cell flattening - traditional and novel approaches.