| Literature DB >> 17544411 |
Futoshi Taura1, Supaart Sirikantaramas, Yoshinari Shoyama, Kazuyoshi Yoshikai, Yukihiro Shoyama, Satoshi Morimoto.
Abstract
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.Entities:
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Year: 2007 PMID： 17544411 DOI： 10.1016/j.febslet.2007.05.043
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124