BACKGROUND: There is little information about the involvement of galectin-9 (Gal-9) in allergic inflammation. Thus, we investigated the role of Gal-9 in asthma model guinea pigs. METHODS: Airway resistance (R(aw)) was measured using a double-flow plethysmograph system. Gal-9 expression in the lung was assessed by Western blot and immunohistochemistry. Eosinophil chemotactic activity was evaluated in a chamber containing a polyvinylpyrolidone-free membrane. Cell apoptosis was analyzed on a flowcytometry with propidium iodide. RESULTS: In cloning guinea pig Gal-9 we identified three isoforms that differ only in the length of their linker peptides, just as with human Gal-9. Guinea pig Gal-9 was found to be a chemoattractant for eosinophils and to promote induction of apoptosis in sensitized but not non-sensitized T lymphocytes. In allergic airway hypersensitivity model, a low level of Gal-9 expression was observed in the nonsensitized/nonchallenged group, but upregulation was detected at 7 h after challenge and sustained up to 24 h. Such upregulation correlated with elevation of eosinophil peroxidase activity but not with increased R(aw). CONCLUSIONS: The present results provide evidence that Gal-9 is not involved in airway hypersensitivity, but is partly involved in prolonged eosinophil accumulation in the lung.
BACKGROUND: There is little information about the involvement of galectin-9 (Gal-9) in allergic inflammation. Thus, we investigated the role of Gal-9 in asthma model guinea pigs. METHODS: Airway resistance (R(aw)) was measured using a double-flow plethysmograph system. Gal-9 expression in the lung was assessed by Western blot and immunohistochemistry. Eosinophil chemotactic activity was evaluated in a chamber containing a polyvinylpyrolidone-free membrane. Cell apoptosis was analyzed on a flowcytometry with propidium iodide. RESULTS: In cloning guinea pigGal-9 we identified three isoforms that differ only in the length of their linker peptides, just as with humanGal-9. Guinea pigGal-9 was found to be a chemoattractant for eosinophils and to promote induction of apoptosis in sensitized but not non-sensitized T lymphocytes. In allergic airway hypersensitivity model, a low level of Gal-9 expression was observed in the nonsensitized/nonchallenged group, but upregulation was detected at 7 h after challenge and sustained up to 24 h. Such upregulation correlated with elevation of eosinophil peroxidase activity but not with increased R(aw). CONCLUSIONS: The present results provide evidence that Gal-9 is not involved in airway hypersensitivity, but is partly involved in prolonged eosinophil accumulation in the lung.
Authors: Ralf Jp van der Valk; Liesbeth Duijts; Nicolas J Timpson; Muhammad T Salam; Marie Standl; John A Curtin; Jon Genuneit; Marjan Kerhof; Eskil Kreiner-Møller; Alejandro Cáceres; Anna Gref; Liming L Liang; H Rob Taal; Emmanuelle Bouzigon; Florence Demenais; Rachel Nadif; Carole Ober; Emma E Thompson; Karol Estrada; Albert Hofman; André G Uitterlinden; Cornélia van Duijn; Fernando Rivadeneira; Xia Li; Sandrah P Eckel; Kiros Berhane; W James Gauderman; Raquel Granell; David M Evans; Beate St Pourcain; Wendy McArdle; John P Kemp; George Davey Smith; Carla Mt Tiesler; Claudia Flexeder; Angela Simpson; Clare S Murray; Oliver Fuchs; Dirkje S Postma; Klaus Bønnelykke; Maties Torrent; Martin Andersson; Patrick Sleiman; Hakon Hakonarson; William O Cookson; Miriam F Moffatt; Lavinia Paternoster; Erik Melén; Jordi Sunyer; Hans Bisgaard; Gerard H Koppelman; Markus Ege; Adnan Custovic; Joachim Heinrich; Frank D Gilliland; Alexander J Henderson; Vincent Wv Jaddoe; Johan C de Jongste Journal: J Allergy Clin Immunol Date: 2013-12-06 Impact factor: 10.793
Authors: Mab P Corrêa; Libnah L Areias; Rebeca D Correia-Silva; Solange C G P D'Ávila; Andréia M Leopoldino; Karin V Greco; Cristiane D Gil Journal: Cells Date: 2021-04-20 Impact factor: 6.600