In vitro effects of adrenomedullin and calcitonin gene related peptide on the release of serotonin and amino acids from rat dorsal spinal cord.

Adrenomedullin (AM) and calcitonin gene-related peptide (CGRP) are structurally related, interact with each others receptors and show overlapping biological activities. Immunoreactivity (IR) and mRNAs along with binding sites for both CGRP and adrenomedullin have been shown in the rat spinal cord. CGRP mediates the transmission of nociceptive information at the spinal cord level and both peptides has shown to induces c-fos expression and accumulation of cAMP in spinal cells. In this study, HPLC methods were used to investigate the effects of AM and CGRP on the basal and K+-evoked release of serotonin, glutamate (Glu), aspartate (Asp), glycine (Gly) and gamma amino butyric acid (GABA) from the slices prepared from the rat spinal cord. Neither CGRP (10(-7) and 10(-6) M) nor AM (10(-7) and 10(-6) M) had significant effects on the basal release of serotonin and the amino acids tested in this study. However, CGRP produced statistically significant increases in the K+-evoked release of Asp and Glu, whereas AM failed to do so. Neither AM nor CGRP (10(-7) and 10(-6) M) showed any significant effects on the K+-evoked release of serotonin, GABA and Gly. Present data suggest that the stimulatory effects of CGRP on the release of Asp and Glu were exerted by distinct types of CGRP receptors.