BACKGROUND: Between January 2005 and April 2006, six patients of influenza A/H5N1 virus infection were reported in Cambodia, all with fatal outcome. OBJECTIVES: We describe the virological findings of these six H5N1 patients in association with clinical and epidemiologic findings. STUDY DESIGN: Broncho-alveolar lavage, nasopharyngeal, throat and rectal swabs and sera were cultured for virus isolation and viral load quantified in clinical specimens by real-time RT-PCR. We compared sequences obtained from different body sites within the same patient to detect viral quasi-species. RESULTS: H5N1 virus strains isolated in Cambodia belong to genotype Z, clade 1 viruses. H5N1 viruses were isolated from serum and rectal swab specimens in two patients. The haemagglutinin gene sequences of the virus in different body sites did not differ. Amino acid substitutions known to be associated with a change in virus binding were not observed. CONCLUSION: The high frequency of virus isolation from serum and faecal swabs highlights that H5N1 is likely to be a disseminated infection in humans and this has implications for antiviral treatment, biosafety in clinical laboratories and on risks for nosocomial and human-to-human transmission. There were no tissue-specific adaptive mutations in the HA gene from viruses isolated from different organs.
BACKGROUND: Between January 2005 and April 2006, six patients of influenza A/H5N1 virus infection were reported in Cambodia, all with fatal outcome. OBJECTIVES: We describe the virological findings of these six H5N1patients in association with clinical and epidemiologic findings. STUDY DESIGN: Broncho-alveolar lavage, nasopharyngeal, throat and rectal swabs and sera were cultured for virus isolation and viral load quantified in clinical specimens by real-time RT-PCR. We compared sequences obtained from different body sites within the same patient to detect viral quasi-species. RESULTS:H5N1 virus strains isolated in Cambodia belong to genotype Z, clade 1 viruses. H5N1 viruses were isolated from serum and rectal swab specimens in two patients. The haemagglutinin gene sequences of the virus in different body sites did not differ. Amino acid substitutions known to be associated with a change in virus binding were not observed. CONCLUSION: The high frequency of virus isolation from serum and faecal swabs highlights that H5N1 is likely to be a disseminated infection in humans and this has implications for antiviral treatment, biosafety in clinical laboratories and on risks for nosocomial and human-to-human transmission. There were no tissue-specific adaptive mutations in the HA gene from viruses isolated from different organs.
Authors: Philippe Buchy; Sirenda Vong; Simon Chu; Jean-Michel Garcia; Tran Tinh Hien; Vo Minh Hien; Mey Channa; Do Quang Ha; Nguyen Van Vinh Chau; Cameron Simmons; Jeremy J Farrar; Malik Peiris; Menno D de Jong Journal: PLoS One Date: 2010-05-27 Impact factor: 3.240
Authors: Angela P Campbell; Jason W Chien; Jane Kuypers; Janet A Englund; Anna Wald; Katherine A Guthrie; Lawrence Corey; Michael Boeckh Journal: J Infect Dis Date: 2010-05-01 Impact factor: 5.226
Authors: Michael C W Chan; Renee W Y Chan; Wendy C L Yu; Carol C C Ho; W H Chui; C K Lo; Kit M Yuen; Y I Guan; John M Nicholls; J S Malik Peiris Journal: Respir Res Date: 2009-10-30
Authors: Philippe Buchy; Mathieu Fourment; Sek Mardy; San Sorn; Davun Holl; Sowath Ly; Sirenda Vong; Vincent Enouf; J S Malik Peiris; Silvie van der Werf Journal: Emerg Infect Dis Date: 2009-10 Impact factor: 6.883
Authors: Charisma Dilantika; Endang R Sedyaningsih; Matthew R Kasper; Magdarina Agtini; Erlin Listiyaningsih; Timothy M Uyeki; Timothy H Burgess; Patrick J Blair; Shannon D Putnam Journal: BMC Infect Dis Date: 2010-01-07 Impact factor: 3.090