PURPOSE: To evaluate the effects of desiccating ocular surface stress on levels of inflammatory cytokines in the corneal epithelium, conjunctiva, and tear fluid of BALB/c and C57BL/6 mice. METHODS: Experimental dry eye (EDE) was created in BALB/c and C57BL/6 mice by cholinergic blockade and exposure to a desiccating environment. Real-time polymerase chain reaction was performed to measure levels of cytokine transcripts. A multiplex immunobead assay was used to measure concentrations of these cytokines in tears. RESULTS: Experimental dryness significantly increased the expression of interleukin (IL)-1alpha, IL-6, and tumor necrosis factor (TNF)-alpha transcripts in the corneal epithelium and conjunctiva of C57BL/6 mice. Strain-specific changes in tear cytokine profiles were observed. C57BL/6 mice had significantly greater tear concentrations of IL-1alpha and TNF-alpha and the Th-1 cytokines IL-2, IL-12, and interferon-gamma in response to desiccating stress than BALB/c mice. The Th-2 cytokines IL-4 and IL-10 were significantly greater in BALB/c tears. CONCLUSIONS: This study indicates that desiccating stress increases levels of certain cytokines in the corneal epithelium and conjunctiva in a strain-dependent fashion and that C57BL/6 mice had greater levels of Th-1 cytokines in their tears, whereas BALB/c mice had a greater increase in Th-2 cytokines.
PURPOSE: To evaluate the effects of desiccating ocular surface stress on levels of inflammatory cytokines in the corneal epithelium, conjunctiva, and tear fluid of BALB/c and C57BL/6 mice. METHODS: Experimental dry eye (EDE) was created in BALB/c and C57BL/6 mice by cholinergic blockade and exposure to a desiccating environment. Real-time polymerase chain reaction was performed to measure levels of cytokine transcripts. A multiplex immunobead assay was used to measure concentrations of these cytokines in tears. RESULTS: Experimental dryness significantly increased the expression of interleukin (IL)-1alpha, IL-6, and tumor necrosis factor (TNF)-alpha transcripts in the corneal epithelium and conjunctiva of C57BL/6 mice. Strain-specific changes in tear cytokine profiles were observed. C57BL/6 mice had significantly greater tear concentrations of IL-1alpha and TNF-alpha and the Th-1 cytokines IL-2, IL-12, and interferon-gamma in response to desiccating stress than BALB/c mice. The Th-2 cytokines IL-4 and IL-10 were significantly greater in BALB/c tears. CONCLUSIONS: This study indicates that desiccating stress increases levels of certain cytokines in the corneal epithelium and conjunctiva in a strain-dependent fashion and that C57BL/6 mice had greater levels of Th-1 cytokines in their tears, whereas BALB/c mice had a greater increase in Th-2 cytokines.
Authors: Yi Wei; Seth P Epstein; Shima Fukuoka; Neil P Birmingham; Xiu-Min Li; Penny A Asbell Journal: Invest Ophthalmol Vis Sci Date: 2011-07-01 Impact factor: 4.799
Authors: Cintia S De Paiva; Kyung-Chul Yoon; Solherny B Pangelinan; Sapa Pham; Larry M Puthenparambil; Eliseu Y Chuang; William J Farley; Michael E Stern; De-Quan Li; Stephen C Pflugfelder Journal: J Inflamm (Lond) Date: 2009-10-31 Impact factor: 4.981