OBJECTIVE: To investigate the effects of human chorionic gonadotropin (hCG) in culture medium on the in vitro maturation (IVM) and subsequent developmental potential of human immature oocytes. DESIGN: Prospective, randomized study. SETTING:Reproductive Medicine Center, First Affiliated Hospital of Wenzhou Medical College, People's Republic of China. PATIENT(S): 62 women with polycystic ovary syndrome (PCOS) undergoing IVM treatment. INTERVENTION(S): Immature oocytes were retrieved from unstimulated ovaries of women with PCOS. We tried three different culture systems in this experiment. In group A, oocytes were cultured in the medium containing M199 + 20% fetal bovine serum (FBS) + 75 mIU/mL recombinant follicle-stimulating hormone (FSH) + 0.5 IU/mLrecombinant hCG. In group B, oocytes were cultured in hCG-free IVM medium (M199 + 20% FBS + 75 mIU/mL recombinant FSH) for 10 hours, then transferred to the same medium as used for group A. In group C, oocytes were cultured only in hCG-free IVM medium. After the oocytes had matured in vitro, fertilization and embryo transfer were performed. MAIN OUTCOME MEASURE(S): Rates of maturation, fertilization, cleavage, implantation, clinical pregnancy, miscarriage, and live birth. RESULT(S): For groups A, B, and C, the maturation rates at 32 and 48 hours were 46.02% and 69.25%; 43.72% and 64.51%; and 51.87% and 67.51%, respectively. Relatively satisfactory clinical results and implantation rates were obtained in all three groups. No statistically significant differences among groups A, B, and C were found in the rates of maturation, fertilization, cleavage, implantation, clinical pregnancy, miscarriage, or live birth. CONCLUSION(S): The results of our study indicated that the addition of hCG to in vitro culture medium did not improve the maturation rate or development potential of immature oocytes. For the IVM and development of immature oocytes from women with PCOS, hCG appears to be unnecessary.
RCT Entities:
OBJECTIVE: To investigate the effects of human chorionic gonadotropin (hCG) in culture medium on the in vitro maturation (IVM) and subsequent developmental potential of human immature oocytes. DESIGN: Prospective, randomized study. SETTING: Reproductive Medicine Center, First Affiliated Hospital of Wenzhou Medical College, People's Republic of China. PATIENT(S): 62 women with polycystic ovary syndrome (PCOS) undergoing IVM treatment. INTERVENTION(S): Immature oocytes were retrieved from unstimulated ovaries of women with PCOS. We tried three different culture systems in this experiment. In group A, oocytes were cultured in the medium containing M199 + 20% fetal bovine serum (FBS) + 75 mIU/mL recombinant follicle-stimulating hormone (FSH) + 0.5 IU/mL recombinant hCG. In group B, oocytes were cultured in hCG-free IVM medium (M199 + 20% FBS + 75 mIU/mL recombinant FSH) for 10 hours, then transferred to the same medium as used for group A. In group C, oocytes were cultured only in hCG-free IVM medium. After the oocytes had matured in vitro, fertilization and embryo transfer were performed. MAIN OUTCOME MEASURE(S): Rates of maturation, fertilization, cleavage, implantation, clinical pregnancy, miscarriage, and live birth. RESULT(S): For groups A, B, and C, the maturation rates at 32 and 48 hours were 46.02% and 69.25%; 43.72% and 64.51%; and 51.87% and 67.51%, respectively. Relatively satisfactory clinical results and implantation rates were obtained in all three groups. No statistically significant differences among groups A, B, and C were found in the rates of maturation, fertilization, cleavage, implantation, clinical pregnancy, miscarriage, or live birth. CONCLUSION(S): The results of our study indicated that the addition of hCG to in vitro culture medium did not improve the maturation rate or development potential of immature oocytes. For the IVM and development of immature oocytes from women with PCOS, hCG appears to be unnecessary.
Authors: David E Reichman; Joseph Politch; Elizabeth S Ginsburg; Catherine Racowsky Journal: J Assist Reprod Genet Date: 2010-04-28 Impact factor: 3.412
Authors: Şafak Hatırnaz; Barış Ata; Ebru Saynur Hatırnaz; Michael Haim Dahan; Samer Tannus; Justin Tan; Seang Lin Tan Journal: Turk J Obstet Gynecol Date: 2018-06-21
Authors: Huixia Yang; Thomas Kolben; Sarah Meister; Corinna Paul; Julia van Dorp; Sibel Eren; Christina Kuhn; Martina Rahmeh; Sven Mahner; Udo Jeschke; Viktoria von Schönfeldt Journal: Biomedicines Date: 2021-12-14