OBJECTIVE: To report a novel approach to fertility preservation by in vitro maturation and embryo cryopreservation after LH surge and ovulation. DESIGN: Case report. SETTING: Two university-based reproductive endocrinology clinics. PATIENT(S): Forty-year-old woman with breast cancer seeking fertility preservation before chemotherapy. INTERVENTION(S): The plan was ovarian stimulation and retrieval of mature oocytes, followed by IVF; however, premature LH surge and ovulation occurred before oocyte retrieval. Because no mature oocyte was recovered, follicles <10 mm in diameter were also aspirated. MAIN OUTCOME MEASURE(S): Recovery of immature oocytes after ovulation, in vitro maturation of these oocytes, and fertilization and vitrification of generated embryos. RESULT(S): Four immature oocytes were recovered, and two matured in vitro. After fertilization with intracytoplasmic sperm injection, embryos progressed to the four-cell stage on day 2 and were vitrified for future use. CONCLUSION(S): This case illustrates that under time constraints, immature oocytes can be recovered after ovulation and used to generate embryos for fertility preservation.
OBJECTIVE: To report a novel approach to fertility preservation by in vitro maturation and embryo cryopreservation after LH surge and ovulation. DESIGN: Case report. SETTING: Two university-based reproductive endocrinology clinics. PATIENT(S): Forty-year-old woman with breast cancer seeking fertility preservation before chemotherapy. INTERVENTION(S): The plan was ovarian stimulation and retrieval of mature oocytes, followed by IVF; however, premature LH surge and ovulation occurred before oocyte retrieval. Because no mature oocyte was recovered, follicles <10 mm in diameter were also aspirated. MAIN OUTCOME MEASURE(S): Recovery of immature oocytes after ovulation, in vitro maturation of these oocytes, and fertilization and vitrification of generated embryos. RESULT(S): Four immature oocytes were recovered, and two matured in vitro. After fertilization with intracytoplasmic sperm injection, embryos progressed to the four-cell stage on day 2 and were vitrified for future use. CONCLUSION(S): This case illustrates that under time constraints, immature oocytes can be recovered after ovulation and used to generate embryos for fertility preservation.
Authors: Min Xu; Asgerally T Fazleabas; Ariella Shikanov; Erin Jackson; Susan L Barrett; Jenny Hirshfeld-Cytron; Sarah E Kiesewetter; Lonnie D Shea; Teresa K Woodruff Journal: Biol Reprod Date: 2010-12-01 Impact factor: 4.285
Authors: Matteo Lambertini; Lucia Del Mastro; Maria C Pescio; Claus Y Andersen; Hatem A Azim; Fedro A Peccatori; Mauro Costa; Alberto Revelli; Francesca Salvagno; Alessandra Gennari; Filippo M Ubaldi; Giovanni B La Sala; Cristofaro De Stefano; W Hamish Wallace; Ann H Partridge; Paola Anserini Journal: BMC Med Date: 2016-01-04 Impact factor: 8.775