Literature DB >> 17516579

Silver- and Coomassie-staining protocols: detection limits and compatibility with ESI MS.

Christiane Winkler1, Katrin Denker, Stefanie Wortelkamp, Albert Sickmann.   

Abstract

Staining protocols for PAGE have to be sensitive and should not impair further MS analysis of selected samples. In this study, the MS compatibility of different silver- and Coomassie-staining protocols with a nano-LC-MS/MS system was systematically elucidated. Altogether, 13 different silver-staining, 1 imidazole-staining and 2 Coomassie-staining protocols were used and compared to each other for their achieved sequence coverage and their detection sensitivity. Three proteins were used as model proteins (bovine serum albumin, rabbit L-lactate dehydrogenase, bovine milk beta-lactoglobulin) in decreasing concentration (12 pmol down to 30 fmol) and different staining protocols were applied. The conclusion of this study is that two silver-staining protocols (Blum, H. et al.,. Electrophoresis 1987, 8, 93-99 and Shevchenko, A. et al.,. Anal. Chem. 1996, 68, 850-858) combine good sequence coverage and good sensitivity and are recommended for nano-LC-MS/MS analysis.

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Year:  2007        PMID： 17516579     DOI： 10.1002/elps.200600670

Source DB:  PubMed          Journal:  Electrophoresis        ISSN： 0173-0835            Impact factor:   3.535


  18 in total

1.  Proteomic and metabolomic analyses of mitochondrial complex I-deficient mouse model generated by spontaneous B2 short interspersed nuclear element (SINE) insertion into NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4) gene.

Authors:  Dillon W Leong; Jasper C Komen; Chelsee A Hewitt; Estelle Arnaud; Matthew McKenzie; Belinda Phipson; Melanie Bahlo; Adrienne Laskowski; Sarah A Kinkel; Gayle M Davey; William R Heath; Anne K Voss; René P Zahedi; James J Pitt; Roman Chrast; Albert Sickmann; Michael T Ryan; Gordon K Smyth; David R Thorburn; Hamish S Scott
Journal:  J Biol Chem       Date:  2012-04-25       Impact factor: 5.157

2.  Inhibition of proinflammatory and innate immune signaling pathways by a cytomegalovirus RIP1-interacting protein.

Authors:  Claudia Mack; Albert Sickmann; David Lembo; Wolfram Brune
Journal:  Proc Natl Acad Sci U S A       Date:  2008-02-19       Impact factor: 11.205

3.  Tyr728 in the kinase domain of the murine kinase suppressor of RAS 1 regulates binding and activation of the mitogen-activated protein kinase kinase.

Authors:  Claudia Sibilski; Thomas Mueller; Laxmikanth Kollipara; René P Zahedi; Ulf R Rapp; Thomas Rudel; Angela Baljuls
Journal:  J Biol Chem       Date:  2013-10-24       Impact factor: 5.157

4.  A stimulatory role for the La-related protein 4B in translation.

Authors:  Katrin Schäffler; Kristina Schulz; Anja Hirmer; Julia Wiesner; Michael Grimm; Albert Sickmann; Utz Fischer
Journal:  RNA       Date:  2010-06-23       Impact factor: 4.942

5.  RioK1, a new interactor of protein arginine methyltransferase 5 (PRMT5), competes with pICln for binding and modulates PRMT5 complex composition and substrate specificity.

Authors:  Gernot Guderian; Christoph Peter; Julia Wiesner; Albert Sickmann; Klaus Schulze-Osthoff; Utz Fischer; Matthias Grimmler
Journal:  J Biol Chem       Date:  2010-11-16       Impact factor: 5.157

6.  Coomassie blue as a near-infrared fluorescent stain: a systematic comparison with Sypro Ruby for in-gel protein detection.

Authors:  R Hussain Butt; Jens R Coorssen
Journal:  Mol Cell Proteomics       Date:  2013-09-16       Impact factor: 5.911

7.  Malignant transformation in a defined genetic background: proteome changes displayed by 2D-PAGE.

Authors:  Stephanie M Pütz; Fotini Vogiatzi; Thorsten Stiewe; Albert Sickmann
Journal:  Mol Cancer       Date:  2010-09-22       Impact factor: 27.401

8.  Ocular proteomics with emphasis on two-dimensional gel electrophoresis and mass spectrometry.

Authors:  Bent Honoré; Henrik Vorum; Nakul Mandal; Steffen Heegaard; Jan Ulrik Prause
Journal:  Biol Proced Online       Date:  2009-12-24       Impact factor: 3.244

9.  BIN2 orchestrates platelet calcium signaling in thrombosis and thrombo-inflammation.

Authors:  Julia Volz; Charly Kusch; Sarah Beck; Michael Popp; Timo Vögtle; Mara Meub; Inga Scheller; Hannah S Heil; Julia Preu; Michael K Schuhmann; Katherina Hemmen; Thomas Premsler; Albert Sickmann; Katrin G Heinze; David Stegner; Guido Stoll; Attila Braun; Markus Sauer; Bernhard Nieswandt
Journal:  J Clin Invest       Date:  2020-11-02       Impact factor: 14.808

10.  In vivo expression and purification of aptamer-tagged small RNA regulators.

Authors:  Nelly Said; Renate Rieder; Robert Hurwitz; Jochen Deckert; Henning Urlaub; Jörg Vogel
Journal:  Nucleic Acids Res       Date:  2009-09-02       Impact factor: 16.971
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