Bing Guan1, Dongdong Zhu, Zhen Dong, Zhanquan Yang. 1. Department of Otorhinolaryngology-Head and Neck Surgery, Clinical Hospital, Yangzhou University, Yangzhou, 225001, China.
Abstract
OBJECTIVE: To detect expression level and distribution of aquaporin 1 (AQP1) in laryngeal carcinoma tissues and try to find out the significance about the mechanism of AQP1 in the invasion of laryngeal carcinoma. METHOD: Twenty cases of laryngeal carcinoma tissues and 15 cases of normal tissues beside tumors were acquired immediately after surgery. The expression and distribution of aquaporin 1 in laryngeal carcinoma were examined by RT-PCR, western blot analysis and immunohistochemical technique. RESULT: AQP1 expresses on the capillary endothelia of laryngeal mucous lamina propria and laryngeal mucous glands of normal tissues and mainly on the capillary endothelia cells, tumor cells and cancer nests of laryngeal carcinoma tissues. AQP1 mRNA and protein expression level in laryngeal tumor tissues is remarkably stronger than that in normal tissues beside tumors. CONCLUSION: The expression of AQP1 was increased in laryngeal carcinoma tissues over normal laryngeal tissues. It suggests that the mechanism of AQP1 in laryngeal carcinoma etiopathogenesis needs to be studied.
OBJECTIVE: To detect expression level and distribution of aquaporin 1 (AQP1) in laryngeal carcinoma tissues and try to find out the significance about the mechanism of AQP1 in the invasion of laryngeal carcinoma. METHOD: Twenty cases of laryngeal carcinoma tissues and 15 cases of normal tissues beside tumors were acquired immediately after surgery. The expression and distribution of aquaporin 1 in laryngeal carcinoma were examined by RT-PCR, western blot analysis and immunohistochemical technique. RESULT: AQP1 expresses on the capillary endothelia of laryngeal mucous lamina propria and laryngeal mucous glands of normal tissues and mainly on the capillary endothelia cells, tumor cells and cancer nests of laryngeal carcinoma tissues. AQP1 mRNA and protein expression level in laryngeal tumor tissues is remarkably stronger than that in normal tissues beside tumors. CONCLUSION: The expression of AQP1 was increased in laryngeal carcinoma tissues over normal laryngeal tissues. It suggests that the mechanism of AQP1 in laryngeal carcinoma etiopathogenesis needs to be studied.
Authors: Chunbo Shao; Wenyue Sun; Marietta Tan; Chad A Glazer; Sheetal Bhan; Xiaoli Zhong; Carole Fakhry; Rajni Sharma; William H Westra; Mohammad O Hoque; Christopher A Moskaluk; David Sidransky; Joseph A Califano; Patrick K Ha Journal: Clin Cancer Res Date: 2011-05-06 Impact factor: 12.531
Authors: Michael L De Ieso; Jinxin V Pei; Saeed Nourmohammadi; Eric Smith; Pak Hin Chow; Mohamad Kourghi; Jennifer E Hardingham; Andrea J Yool Journal: Sci Rep Date: 2019-09-02 Impact factor: 4.379