Literature DB >> 17510811

A two-color acid-free cartilage and bone stain for zebrafish larvae.

M B Walker1, C B Kimmel.   

Abstract

Traditionally, cartilage is stained by alcian blue using acidic conditions to differentiate tissue staining. The acidic conditions are problematic when one wishes to stain the same specimen for mineralized bone with alizarin red, because acid demineralizes bone, which negatively affects bone staining. We have developed an acid-free method to stain cartilage and bone simultaneously in zebrafish larvae. This method has the additional advantage that PCR genotyping of stained specimens is possible.

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Year:  2007        PMID： 17510811     DOI： 10.1080/10520290701333558

Source DB:  PubMed          Journal:  Biotech Histochem        ISSN： 1052-0295            Impact factor:   1.718


  248 in total

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Authors:  Craig T Miller; Mary E Swartz; Patricia A Khuu; Macie B Walker; Johann K Eberhart; Charles B Kimmel
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