Literature DB >> 17509790

Pyrosequencing of a recA gene variable region for Burkholderia cepacia complex genomovar identification.

Robert Slinger1, Liying Yan, Rene Myers, Karam Ramotar, Melissa St Denis, Shawn D Aaron.   

Abstract

We developed an assay to identify Burkholderia cepacia complex genomovars by Pyrosequencing of a variable recA gene segment. Fifteen reference strains and 30 clinical isolates of B. cepacia complex were sequenced. Full 77-base pair target sequences were obtained from 44 of the 45 isolates, and BLAST queries of the sequences correctly identified the genomovar of these 44 isolates. Three Burkholderia multivorans isolates were identified as B. multivorans/Burkholderia ambifaria, indicating that additional identification methods may be needed for some B. multivorans strains.

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Year:  2007        PMID： 17509790     DOI： 10.1016/j.diagmicrobio.2007.02.011

Source DB:  PubMed          Journal:  Diagn Microbiol Infect Dis        ISSN： 0732-8893            Impact factor:   2.803


  5 in total

1.  Performances of the Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry system for rapid identification of bacteria in routine clinical microbiology.

Authors:  Damien Dubois; Marion Grare; Marie-Françoise Prere; Christine Segonds; Nicole Marty; Eric Oswald
Journal:  J Clin Microbiol       Date:  2012-05-16       Impact factor: 5.948

2.  Burkholderia cepacia complex in Serbian patients with cystic fibrosis: prevalence and molecular epidemiology.

Authors:  Z V Vasiljevic; K Novovic; M Kojic; P Minic; A Sovtic; S Djukic; B Jovcic
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2016-05-13       Impact factor: 3.267

3.  Pyrosequencing assay to rapidly detect clarithromycin resistance mutations in Canadian Helicobacter pylori isolates.

Authors:  R Slinger; L Yan; F Chan; K Forward; G Cooper-Lesins; L Best; D Haldane; S Veldhuyzen van Zanten
Journal:  Can J Gastroenterol       Date:  2009-09       Impact factor: 3.522

4.  Prevalence and Identification of Burkholderia pseudomallei and Near-Neighbor Species in the Malabar Coastal Region of India.

Authors:  Bhavani V Peddayelachagiri; Soumya Paul; Sowmya Nagaraj; Madhurjya Gogoi; Murali H Sripathy; Harsh V Batra
Journal:  PLoS Negl Trop Dis       Date:  2016-09-15

5.  Draft Genome Sequence of Burkholderia cepacia ATCC 17759, a Polyhydroxybutyrate-Co-Valerate Copolymer-Producing Bacterium.

Authors:  Casey Crooks; Jonathan M Palmer; Daniel L Lindner
Journal:  Genome Announc       Date:  2018-04-26
  5 in total

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