Literature DB >> 17507513

Increased detection of respiratory syncytial virus, influenza viruses, parainfluenza viruses, and adenoviruses with real-time PCR in samples from patients with respiratory symptoms.

Alma C van de Pol1, Anton M van Loon, Tom F W Wolfs, Nicolaas J G Jansen, Monique Nijhuis, Els Klein Breteler, Rob Schuurman, John W A Rossen.   

Abstract

Respiratory samples (n = 267) from hospitalized patients with respiratory symptoms were tested by real-time PCR, viral culture, and direct immunofluorescence for respiratory syncytial virus, influenza virus, parainfluenza viruses, and adenoviruses. Compared with conventional diagnostic tests, real-time PCR increased the diagnostic yields for these viruses from 24% to 43% and from 3.5% to 36% for children and adults, respectively.

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Year:  2007        PMID： 17507513      PMCID： PMC1932984          DOI： 10.1128/JCM.00848-07

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  10 in total

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Authors:  L J van Elden; M Nijhuis; P Schipper; R Schuurman; A M van Loon
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4.  Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4.

Authors:  Kate E Templeton; Sitha A Scheltinga; Matthias F C Beersma; Aloys C M Kroes; Eric C J Claas
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5.  Prospective study of respiratory viral infections in pediatric hemopoietic stem cell transplantation patients.

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6.  Lack of sensitivity of rapid antigen tests for the diagnosis of respiratory syncytial virus infection in adults.

Authors:  Aida E Casiano-Colón; Barbara B Hulbert; Theodor K Mayer; Edward E Walsh; Ann R Falsey
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7.  Applicability of a real-time quantitative PCR assay for diagnosis of respiratory syncytial virus infection in immunocompromised adults.

Authors:  L J R van Elden; A M van Loon; A van der Beek; K A W Hendriksen; A I M Hoepelman; M G J van Kraaij; P Schipper; M Nijhuis
Journal:  J Clin Microbiol       Date:  2003-09       Impact factor: 5.948

8.  Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection.

Authors:  Alma C van de Pol; Tom F W Wolfs; Nicolaas J G Jansen; Anton M van Loon; John W A Rossen
Journal:  Crit Care       Date:  2006       Impact factor: 9.097

9.  Frequent detection of respiratory viruses in adult recipients of stem cell transplants with the use of real-time polymerase chain reaction, compared with viral culture.

Authors:  Marian G J van Kraaij; Leontine J R van Elden; Anton M van Loon; Karin A W Hendriksen; Laurens Laterveer; Adriaan W Dekker; Monique Nijhuis
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Journal:  J Clin Virol       Date:  2004-03       Impact factor: 3.168
  10 in total
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2.  Evaluation of high-throughput sequencing for identifying known and unknown viruses in biological samples.

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3.  Molecular characterization of human parainfluenza virus type 1 in infants attending Mbagathi District Hospital, Nairobi, Kenya: a retrospective study.

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5.  A systemic neutrophil response precedes robust CD8(+) T-cell activation during natural respiratory syncytial virus infection in infants.

Authors:  Michaël V Lukens; Alma C van de Pol; Frank E J Coenjaerts; Nicolaas J G Jansen; Vera M Kamp; Jan L L Kimpen; John W A Rossen; Laurien H Ulfman; Carline E A Tacke; Marco C Viveen; Leo Koenderman; Tom F W Wolfs; Grada M van Bleek
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6.  Comparison of polyurethane foam to nylon flocked swabs for collection of secretions from the anterior nares in performance of a rapid influenza virus antigen test in a pediatric emergency department.

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7.  Detection of multiple respiratory pathogens during primary respiratory infection: nasal swab versus nasopharyngeal aspirate using real-time polymerase chain reaction.

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9.  Comparison of conventional and molecular detection of respiratory viruses in hematopoietic cell transplant recipients.

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10.  Estimating sensitivity of laboratory testing for influenza in Canada through modelling.

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