Stepped potential microcoulometry of ferritin.

A coulometric method for measuring the redox potentials of ferritins and the rates of reduction of the iron core is reported. The half-reduction potential, Eo', of the protein is determined from the coulomb-applied potential curve. This curve is obtained from the difference between the curves for the mediator plus protein and the mediator alone. The coulomb-applied potential curves are generated by stepping the potential in increments over the region of interest and measuring the charged passed following each step. The method is reasonably fast, enabling the redox potential to be determined in 1 day, and gives Eo', values for mediators and the protein that are in good agreement with literature values.