| Literature DB >> 17502673 |
Terence P McDonald1, Richard J Sugrue.
Abstract
The respiratory syncytial virus (RSV) fusion (F) protein is synthesized as an inactive precursor (F0), which subsequently undergoes post-translational cleavage to give the disulphide bond-linked F1 and F2 subunits. The methodology detailing the use of two-dimensional electrophoresis, endoglycosidases, and alpha-mannosidase inhibitors, as applied to investigating F protein glycan maturation, is given. Examples are used to show how this methodology was used to provide evidence for glycan heterogeneity within the mature F protein.Entities:
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Year: 2007 PMID： 17502673 DOI： 10.1007/978-1-59745-393-6_7
Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745