| Literature DB >> 17498821 |
You Wang1, Jiangying Liu, Hong Zhao, Wenqing Lü, Jun Zhao, Lin Yang, Ning Li, Xiaojuan Du, Yang Ke.
Abstract
1A6/DRIM is a nucleolar protein with a nucleolar targeting sequence in its 3'-terminus. Bioinformatic analysis indicated that human 1A6/DRIM shares 23% identity and 43% similarity with yeast Utp20, which has been reported as a component of U3 snoRNA protein complex and has been implicated in 18S rRNA processing. In the present study, we found, by utilizing RT-PCR with RNA extracted from anti-1A6/DRIM immunoprecipitates and Northern blotting, that 1A6/DRIM is associated with U3 snoRNA. Pulse-chase labeling assays showed that silencing of 1A6/DRIM expression in HeLa cells resulted in a delayed 18S rRNA processing. Furthermore, immunoprecipitations revealed that 1A6/DRIM was also associated with fibrillarin, another U3 RNP component in HeLa cells. These results indicate that 1A6/DRIM is involved in 18S rRNA processing and is the bona fide mammalian Utp20.Entities:
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Year: 2007 PMID： 17498821 DOI： 10.1016/j.bbamcr.2007.04.002
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002